High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes

This article describes a method for high-throughput competition assays using a bioluminescent strain of . This method is based on the use of the luminescent indicator strain EGDe The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of in mixed culture...

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Bibliographic Details
Published in:	Frontiers in microbiology Vol. 9; p. 1883
Main Authors:	El Kheir, Sara M, Cherrat, Lamia, Awussi, Ahoefa A, Ramia, Nancy E, Taha, Samir, Rahman, Abdur, Passerini, Delphine, Leroi, Françoise, Petit, Jeremy, Mangavel, Cécile, Revol-Junelles, Anne-Marie, Borges, Frédéric
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media 17-08-2018 Frontiers Media S.A
Subjects:	Biodiversity bioluminescence biopreservation competition Ecology, environment Ecosystems Genetics high-throughput screening assays Life Sciences Listeria monocytogenes Microbiology mixed culture Symbiosis bioprotection high-throughput screening assays mixed culture bioluminescence biopreservation Listeria monocytogenes competition antibacterial activities
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Summary:	This article describes a method for high-throughput competition assays using a bioluminescent strain of . This method is based on the use of the luminescent indicator strain EGDe The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of in mixed cultures. To this aim, luminescence kinetics were converted into a single numerical value, called the Luminescence Disturbance Indicator (LDI), which takes into account growth inhibition phenomena resulting in latency increase, decrease in the luminescence rate, or reduction of the maximum luminescence. The LDI allows to automatically and simultaneously handle multiple competition assays which are required for high-throughput screening (HTS) approaches. The method was applied to screen a collection of 1810 strains isolated from raw cow's milk in order to identify non-acidifying strains with anti- bioprotection properties. This method was also successfully used to identify anti- candidates within a collection of , a species where antagonism was previously described as non-diffusible and requiring cell-to-cell contact. In conclusion, bioluminescent can be used in HTS to identify strains with anti- bioprotection properties, irrespectively of the inhibition mechanism.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Djamel Drider, Lille University of Science and Technology, France This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology Reviewed by: Luis Augusto Nero, Universidade Federal de Viçosa, Brazil; Alessandra De Cesare, Università degli Studi di Bologna, Italy
ISSN:	1664-302X 1664-302X
DOI:	10.3389/fmicb.2018.01883