Urine cell-free microRNA as biomarkers for transitional cell carcinoma

Urine cell-free microRNA as biomarkers for transitional cell carcinoma

MicroRNA (miRNA) are short nucleotide strands with a regulatory function in the cell. Several miRNAs have been shown to be useful as biomarkers for different neoplasms. The aim of this project was to assess whether levels of miRNA in cell free urine could be used as a biomarker in transitional cell...

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Published in:BMC research notes Vol. 10; no. 1; p. 641
Main Authors: Geva, Gil A, Gielchinsky, Ilan, Aviv, Nina, Max, Klaas E A, Gofrit, Ofer N, Gur-Wahnon, Devorah, Ben-Dov, Iddo Z
Format: Journal Article
Language:English
Published: England BioMed Central Ltd 29-11-2017
BioMed Central
BMC
Subjects:
Adenocarcinoma
Biological markers
Biomarkers
Biomarkers, Tumor - urine
Carcinoma, Transitional Cell - diagnosis
Carcinoma, Transitional Cell - urine
Case-Control Studies
Cell-Free System
DNA, Complementary - genetics
Extracellular RNA
High-Throughput Nucleotide Sequencing
Humans
MicroRNA
MicroRNAs - urine
Research Note
Transitional cell carcinoma
Urologic Neoplasms - diagnosis
Urologic Neoplasms - urine
Urothelial carcinoma
Biomarkers
microRNA
Extracellular RNA
Transitional cell carcinoma
Urothelial carcinoma
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Summary:MicroRNA (miRNA) are short nucleotide strands with a regulatory function in the cell. Several miRNAs have been shown to be useful as biomarkers for different neoplasms. The aim of this project was to assess whether levels of miRNA in cell free urine could be used as a biomarker in transitional cell carcinoma (TCC). cDNA libraries were produced based on small RNAs in urine samples of fourteen TCC patients and twenty healthy volunteers. Resulting reads were deep sequenced on Illumina HiSeq sequencer with the intent of characterizing cell free urine miRNA profiles. A statistically significant difference was found for a single miRNA; miR-210 was > sixfold higher in the TCC group compared to the control group. Furthermore, we were able to produce a diagnostic score by summing of standardized levels of overexpressed miRNA. This score was considerably higher in TCC patients with a sensitivity of 0.93, specificity of 0.76 and negative predictive value > 0.97.
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ISSN:1756-0500
1756-0500
DOI:10.1186/s13104-017-2950-9