Bacterial community in the rhizosphere and rhizoplane of wild type and transgenic eucalyptus

The rhizosphere is a niche exploited by a wide variety of bacteria. The expression of heterologous genes by plants might become a factor affecting the structure of bacterial communities in the rhizosphere. In a greenhouse experiment, the bacterial community associated to transgenic eucalyptus, carry...

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Bibliographic Details
Published in:	World journal of microbiology & biotechnology Vol. 25; no. 6; pp. 1065 - 1073
Main Authors:	Andreote, Fernando D., Rossetto, Priscilla B., Mendes, Rodrigo, Avila, Luciana A., Labate, Carlos A., Pizzirani-Kleiner, Aline A., Azevedo, João L., Araújo, Welington L.
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-06-2009 Springer Springer Nature B.V
Subjects:	Actinobacteria Actinomycetales Applied Microbiology Bacillaceae Bacteria Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Cellulose Ecology Environmental Engineering/Biotechnology Eucalyptus Flowers & plants Fundamental and applied biological sciences. Psychology Genes Genotypes Life Sciences Microbiology Mycobacterium Original Paper Principal components analysis Rhizosphere Ribosomal DNA Seeds Studies Transgenic plants Variance analysis Microbial ecology Principal components analysis Plant–bacteria interactions DGGE Interaction Ecology Plant―bacteria interactions Eucalyptus Rhizosphere Dicotyledones Angiospermae Myrtaceae Bacteria Spermatophyta Microorganism Transgenesis Microbial community Principal component analysis
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Summary:	The rhizosphere is a niche exploited by a wide variety of bacteria. The expression of heterologous genes by plants might become a factor affecting the structure of bacterial communities in the rhizosphere. In a greenhouse experiment, the bacterial community associated to transgenic eucalyptus, carrying the Lhcb1-2 genes from pea (responsible for a higher photosynthetic capacity), was evaluated. The culturable bacterial community associated to transgenic and wild type plants were not different in density, and the Amplified Ribosomal DNA Restriction Analysis (ARDRA) typing of 124 strains revealed dominant ribotypes representing the bacterial orders Burkholderiales , Rhizobiales , and Actinomycetales , the families Xanthomonadaceae , and Bacillaceae , and the genus Mycobacterium . Principal Component Analysis based on the fingerprints obtained by culture-independent Denaturing Gradient Gel Electrophoresis analysis revealed that Alphaproteobacteria , Betaproteobacteria and Actinobacteria communities responded differently to plant genotypes. Similar effects for the cultivation of transgenic eucalyptus to those observed when two genotype-distinct wild type plants are compared.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0959-3993 1573-0972
DOI:	10.1007/s11274-009-9990-9