Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice

Non-diabetic individuals use hormones like insulin to improve muscle strength and performance. However, as insulin also leads the liver and the adipose tissue to an anabolic state, the purpose of this study was to investigate the effects of insulin on liver metabolism in trained non-diabetic Swiss m...

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Bibliographic Details
Published in:	Brazilian journal of medical and biological research Vol. 52; no. 2; p. e7637
Main Authors:	Pereira, V A R, Vedovelli, K S, Muller, G Y, Depieri, Y F, Avelar, D H C G, de Amo, A H E, Jimenes, D R, Martins, J N L, Silvério, A C, Gomes, C R G, Godoi, V A F, Pedrosa, M M D
Format:	Journal Article
Language:	English
Published:	Brazil Associacao Brasileira de Divulgacao Cientifica (ABDC) 01-01-2019 Revista Brasileira de Pesquisas Medicas Associação Brasileira de Divulgação Científica
Subjects:	Adipose tissue Adiposity Alanine BIOLOGY Body weight Body weight gain Bodybuilders Diabetes Diabetes mellitus Gastrocnemius muscle Gluconeogenesis Glucose Glucose metabolism Glutamine Glycerol Glycogen Health aspects Hormones Insulin Laboratory rats Lactates Lactic acid Liver MEDICINE, RESEARCH & EXPERIMENTAL Metabolism Muscle strength Perfusion Peritoneum Physical training Polysaccharides Pyruvic acid Training Weight training Brazil Training Muscle strength Adiposity Liver Gluconeogenesis
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Summary:	Non-diabetic individuals use hormones like insulin to improve muscle strength and performance. However, as insulin also leads the liver and the adipose tissue to an anabolic state, the purpose of this study was to investigate the effects of insulin on liver metabolism in trained non-diabetic Swiss mice. The mice were divided into four groups: sedentary treated with saline (SS) or insulin (SI) and trained treated with saline (TS) or insulin (TI). Training was made in a vertical stair, at 90% of the maximum load, three times per week. Insulin (0.3 U/kg body weight) or saline were given intraperitoneally five times per week. After eight weeks, tissue and blood were collected and in situ liver perfusion with glycerol+lactate or alanine+glutamine (4 mM each) was carried out. The trained animals increased their muscle strength (+100%) and decreased body weight gain (-11%), subcutaneous fat (-42%), mesenteric fat (-45%), and peritoneal adipocyte size (-33%) compared with the sedentary groups. Insulin prevented the adipose effects of training (TI). The gastrocnemius muscle had greater density of muscle fibers (+60%) and less connective tissue in the trained groups. Liver glycogen was increased by insulin (SI +40% and TI +117%), as well as liver basal glucose release (TI +40%). Lactate and pyruvate release were reduced to a half by training. The greater gluconeogenesis from alanine+glutamine induced by training (TS +50%) was reversed by insulin (TI). Insulin administration had no additional effect on muscle strength and reversed some of the lipolytic and gluconeogenic effects of the resistance training. Therefore, insulin administration does not complement training in improving liver glucose metabolism.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0100-879X 1414-431X 1414-431X 1678-4510
DOI:	10.1590/1414-431X20187637