Bioglass/collagen scaffolds combined with bone marrow stromal cells on bone healing in an experimental model in cranial defects in rats

Bioglass/collagen scaffolds combined with bone marrow stromal cells on bone healing in an experimental model in cranial defects in rats

This study aimed to develop bone regenerative therapeutic strategies, based on the addition of bone marrow stromal cells (BMSC) on bioglass/collagen (BG/COL) scaffolds. For this purpose, an in vivo study was conducted using tissue response of the BG/COL scaffolds combined with BMSC in a critical-siz...

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Bibliographic Details
Published in:Journal of biomaterials applications Vol. 37; no. 9; pp. 1632 - 1644
Main Authors: Kido, HW, Gabbai-Armelin, PR, Magri, AMP, Fernandes, KR, Cruz, MA, Santana, AF, Caliari, HM, Parisi, JR, Avanzi, IR, Daguano, JKMB, Granito, RN, Fortulan, CA, Rennó, ACM
Format: Journal Article
Language:English
Published: London, England SAGE Publications 01-04-2023
Subjects:
Animals
Bone Marrow Cells
Bone Regeneration
Collagen - pharmacology
Mesenchymal Stem Cells
Models, Theoretical
Osteogenesis
Rats
Rats, Wistar
Tissue Engineering - methods
Tissue Scaffolds
collagen
critical bone defect
tissue engineering
Bioglass
bone repair
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Summary:This study aimed to develop bone regenerative therapeutic strategies, based on the addition of bone marrow stromal cells (BMSC) on bioglass/collagen (BG/COL) scaffolds. For this purpose, an in vivo study was conducted using tissue response of the BG/COL scaffolds combined with BMSC in a critical-size defects. Wistar rats were submitted to the surgical procedure to perform the cranial critical size bone defects and distributed in four groups (20 animals per group): Control Group (CG) (rats submitted to the cranial bone defect surgery without treatment), Bioglass Group (BG) (rats treated with BG), BG/COL Group (rats treated with BG/COL) and Bioglass/Collagen and BMSC Group (BG/COL/BMSC) (rats treated with BG/COL scaffolds enriched with BMSCs). Animals were euthanized 15 and 30 days after surgery. Scanning electron microscopy, histopathological and immunohistochemistry analysis were used. SEM analysis demonstrated that porous scaffolds were obtained, and Col fibers were successfully impregnated to BG matrices. The implantation of the BMSC on BG/COL based scaffolds was effective in stimulating newly bone formation and produced an increased immunoexpression of markers related to the bone repair. These results highlight the potential of BG/COL scaffolds and BMSCs to be used as a therapeutic approach for bone regeneration. Graphical Abstract
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ISSN:0885-3282
1530-8022
DOI:10.1177/08853282231163752