Search Results - "Austen, Douglas A."

Crystal Structure of Aurora-2, an Oncogenic Serine/Threonine Kinase by Cheetham, Graham M T, Knegtel, Ronald M A, Coll, Joyce T, Renwick, Suzanne B, Swenson, Lora, Weber, Peter, Lippke, Judith A, Austen, Douglas A

“…Aurora-2 is a key member of a closely related subgroup of serine/threonine kinases that plays important roles in the completion of essential mitotic events…”
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High-throughput screening for soluble recombinant expressed kinases in Escherichia coli and insect cells by Chambers, Stephen P, Austen, Douglas A, Fulghum, John R, Kim, Walter M

“…We have constructed a dual expression vector for the production of recombinant proteins in both Escherichia coli and insect cells. In this vector, the…”
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E. coli and insect cell expression, automated purification and quantitative analysis by Chambers, Stephen P, Fulghum, John R, Austen, Douglas A, Lu, Fan, Swalley, Susanne E

“…The production of recombinant proteins usually involves the exploration of a wide variety of expression and purification methodologies in the pursuit of a…”
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Development of a protease production platform for structure-based drug design by Fox, Ted, Brennan, Debra, Austen, Douglas A, Swalley, Susanne E, Coll, Joyce T, Raybuck, Scott A, Chambers, Stephen P

“…Structure-based drug design (SBDD) has played an integral role in the development of highly specific, potent protease inhibitors resulting in a number of drugs…”
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Effect of Promoters and Signal Sequences on the Production of Secreted HIV-1 gp120 Protein in the Baculovirus System by Golden, Alex, Austen, Douglas A., van Schravendijk, M.R., Sullivan, Barbara J., Kawasaki, Ernest S., Osburne, Marcia S.

“…We compared insect cell production levels of secreted HIV-1 gp120 glycoprotein encoded by five different baculovirus expression constructs. Combinations…”
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Construction and characterization of a radio-iodinatable mutant of recombinant human CD4 by Ferrer, Marc, Godbout, Kevin L, Sullivan, Barbara J, Austen, Douglas A, Sanderson, Christine T, Kelley, Kevin C, Osburne, Marcia S, Harrison, Stephen C, van Schravendijk, Marie R

“…Recombinant soluble human CD4 (rsCD4) has been used in iodinated form to study the interaction of CD4 with its ligands. However, the utility of [ 125 I ]-rsCD4…”
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Structure of GSK3β reveals a primed phosphorylation mechanism by ter Haar, Ernst, Coll, Joyce T, Austen, Douglas A, Hsiao, Hsun-Mei, Swenson, Lora, Jain, Jugnu

“…GSK3β was identified as the kinase that phosphorylates glycogen synthase but is now known to be involved in multiple signaling pathways. GSK3β prefers prior…”
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Carcinoembryonic Antigens: Alternative Splicing Accounts for the Multiple mRNAs That Code for Novel Members of the Carcinoembryonic Antigen Family by Barnett, Thomas R., Kretschmer, Axel, Austen, Douglas A., Goebel, Scott J., Hart, John T., Elting, James J., Kamarck, Michael E.

“…The recent cloning of complete cDNAs encoding carcinoembryonic antigen (CEA) and nonspecific cross-reacting antigen has revealed the existence of a new gene…”
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Regulation of sCD4-183 gene expression from phage-T7-based vectors in Escherichia coli by Kelley, Kevin C., Huestis, Kevin J., Austen, Douglas A., Sanderson, Christine T., Donoghue, Mary A., Stickel, Shawn K., Kawasaki, Ernest S., Osburne, Marcia S.

“…In this paper, we describe various parameters affecting the regulation of expression of the sCD4-183 gene, encoding the 183-amino-acid soluble human two-domain…”
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