Search Results - "Aunins, J"

Use of adenoviral vectors as veterinary vaccines by FERREIRA, T. B, ALVES, P. M, AUNINS, J. G, CARRONDO, M. J. T

“…Vaccines are the most effective and inexpensive prophylactic tool in veterinary medicine. Ideally, vaccines should induce a lifelong protective immunity…”
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Computational and experimental investigation of flow and fluid mixing in the roller bottle bioreactor by Unger, D. R., Muzzio, F. J., Aunins, J. G., Singhvi, R.

“…The fully three‐dimensional velocity field in a roller bottle bioreactor is simulated for two systems (creeping flow and inertial flow conditions) using a…”
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Quantitative Studies of Cell-Bubble Interactions and Cell Damage at Different Pluronic F-68 and Cell Concentrations by Ma, Ningning, Chalmers, Jeffery J., Auniņš, John G., Zhou, Weichang, Xie, Liangzhi

“…Pluronic F‐68 (PF‐68) is routinely used as a shear‐protection additive in mammalian cell cultures. However, most previous studies of its shear protection…”
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Phosphate feeding improves high-cell-concentration NS0 myeloma culture performance for monoclonal antibody production by deZengotita, Vivian M., Miller, William M., Aunins, John G., Zhou, Weichang

“…Phosphorus depletion was identified in high‐cell‐concentration fed‐batch NS0 myeloma cell cultures producing a humanized monoclonal antibody (MAb). In these…”
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Fluid mechanics, cell distribution, and environment in CellCube bioreactors by AUNINS, John G, BADER, Brett, CAOLA, Anthony, GRIFFITHS, Janet, KATZ, Maayan, LICARI, Peter, RAM, Kripa, RANUCCI, Colette S, ZHOU, Weichang

“…Cultivation of MRC-5 cells and attenuated hepatitis A virus (HAV) for the production of VAQTA, an inactivated HAV vaccine (1), is performed in the CellCube…”
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Serum-free suspension cultivation of PER.C6® cells and recombinant adenovirus production under different pH conditions by Xie, Liangzhi, Pilbrough, Warren, Metallo, Christian, Zhong, Tanya, Pikus, Lana, Leung, John, Auniņš, John G., Zhou, Weichang

“…PER.C6® cell growth, metabolism, and adenovirus production were studied in head‐to‐head comparisons in stirred bioreactors under different pH conditions. Cell…”
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Large-scale mammalian cell culture by Hu, Wei-Shou, Aunins, John G

“…Mammalian cell culture continues to draw major research efforts. A great deal of progress has recently been made in cellular physiology, especially in factors…”
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Two-dimensional versus three-dimensional culture systems: Effects on growth and productivity of BHK cells by Alves, P. M., Moreira, J. L., Rodrigues, J. M., Aunins, J. G., Carrondo, M. J. T.

“…The influence of surface growth (two‐dimensional microcarriers) and three‐dimensional growth (aggregates and macroporous supports) in agitated, suspended batch…”
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Changes in animal cell natural aggregates in suspended batch cultures by MOREIRA, J. L, ALVES, P. M, AUNINS, J. G, CARRONDO, M. J. T

“…Some anchorage-dependent animal cells can form natural aggregates in stirred tanks. Baby hamster kidney (BHK) natural aggregates are described and…”
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Monoclonal Antibody Process Development Using Medium Concentrates by Bibila, Theodora A., Ranucci, Colette S., Glazomitsky, Konstantin, Buckland, Barry C., Aunins, John G.

“…A fed‐batch process using concentrated medium was evaluated for its ability to improve cell culture longevity and final monoclonal antibody (MAb) titers for…”
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Comparative analysis of the effects of packaging signal, transgene orientation, promoters, polyadenylation signals, and E3 region on growth properties of first-generation adenoviruses by Youil, Rima, Toner, Timothy J, Su, Qin, Casimiro, Danilo, Shiver, John W, Chen, Ling, Bett, Andrew J, Rogers, Bethany M, Burden, Eric C, Tang, Aimin, Chen, Michelle, Emini, Emilio A, Kaslow, David C, Aunins, John G, Altaras, Nedim E

“…First-generation adenovectors have been developed for gene therapy and vaccine applications. The construction of these adenovectors has entailed the use of…”
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Serum-free and serum-containing media for growth of suspended BHK aggregates in stirred vessels by Moreira, J.L., Alves, P.M., Feliciano, A.S., Aunins, J.G., Carrondo, M.J.T.

“…Baby hamster kidney (BHK) cells were grown in stirred spinner flasks at 45 rpm, using both serum-containing and serum-free media. In both studies natural…”
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Studies of baby hamster kidney natural cell aggregation in suspended batch cultures by Moreira, J L, Alves, P M, Rodrigues, J M, Cruz, P E, Aunins, J G, Carrondo, M J

“…Microcarrier cultures of animal cells of industrial relevance are known to shed aggregates into the suspension phase. For a BHK cell line, which is known to be…”
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Development, preparation, and testing of VAQTA®, a highly purified hepatitis A vaccine by Hagen, A., Aunins, J., DePhillips, P., Oswald, C. B., Hennessey Jr, J. P., Lewis, J., Armstrong, M., Oliver, C., Orella, C., Buckland, B., Sitrin, R.

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Investigation of NS0 cell metabolic behavior in monoclonal antibody producing clones by Bibila, T, Ranucci, C, Glazomitsky, K, Buckland, B, Aunins, J

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Repeated-batch cultures of Baby Hamster Kidney cell aggregates in stirred vessels by MOREIRA, J. L, FELICIANO, A. S, SANTANA, P. C, CRUZ, P. E, AUNINS, J. G, CARRONDO, M. J. T

“…Natural aggregates of Baby Hamster Kidney cells were grown in stirred vessels operated as repeated-batch cultures during more than 600 hours. Different…”
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Large scale production of recombinant mouse and rat growth hormone by fed-batch GS-NS0 cell cultures by Zhou, Weichang, Bibila, T, Glazomitsky, K, Montalvo, J, Chan, C, DiStefano, D, Munshi, S, Robinson, D, Buckland, B, Aunins, J

“…Investigations of biological effects of prolonged elevation of growth hormone in animals such as mice and rats require large amounts of mouse and rat growth…”
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Experimental collision efficiencies of polymer-flocculated animal cells by Aunins, J.G, Wang, D.I.C

“…The determination of particle collision kinetics is useful to decouple the effects of process parameters on individual events in flocculation. This paper…”
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Formation and disruption of animal cell aggregates in stirred vessels: Mechanisms and kinetic studies by Moreira, JoséL., Cruz, Pedro E., Santana, Paula C., Aunins, John G., Carrondo, Manuel J.T.

“…The kinetics and mechanisms of aggregate formation and disruption have been investigated for BHK natural aggregates grown in 250 cm 3 Corning spinner flasks…”
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Dissolution of carboxylic acids. III: The effect of polyionizable buffers by Aunins, J G, Southard, M Z, Myers, R A, Himmelstein, K J, Stella, V J

“…The dissolution behavior of three carboxylic acids of variable aqueous solubility but with approximately equal pKa values into aqueous buffered solutions has…”
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