cDNA clones reveal differences between human glial and endothelial cell platelet-derived growth factor A-chains

Human platelet-derived growth factor (PDGF) is a potent mitogenic polypeptide which is believed to be a heterodimer of A- and B-chains stabilized by interchain disulphide bonds. The B-chain of PDGF is encoded by the c-sis gene, the normal cellular homologue of the transforming gene of the simian sar...

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Bibliographic Details
Published in:	Nature (London) Vol. 328; no. 6131; pp. 619 - 621
Main Authors:	Tong, Benton D, Auer, David E, Jaye, Michael, Kaplow, June M, Ricca, George, McConathy, Evelyn, Drohan, William, Deuel, Thomas F
Format:	Journal Article
Language:	English
Published:	London Nature Publishing 13-08-1987
Subjects:	amino acid sequence Animals Base Sequence Biological and medical sciences Cell Line DNA - genetics endothelium Endothelium - analysis Fibroblast Growth Factors - pharmacology Fundamental and applied biological sciences. Psychology Genes. Genome Humans Macromolecular Substances man Molecular and cellular biology Molecular genetics Neuroglia - analysis Nucleic Acid Hybridization nucleotide sequence platelet-derived growth factor Platelet-Derived Growth Factor - genetics RNA, Messenger - genetics Umbilical Veins Human C terminal-Sequence Endothelial cell Complementary DNA Gene Transformed cell Normal Comparative study Platelet derived growth factor
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Summary:	Human platelet-derived growth factor (PDGF) is a potent mitogenic polypeptide which is believed to be a heterodimer of A- and B-chains stabilized by interchain disulphide bonds. The B-chain of PDGF is encoded by the c-sis gene, the normal cellular homologue of the transforming gene of the simian sarcoma virus (SSV). cDNA clones of the B-chain from both normal and transformed cells have mutually consistent DNA sequences. Recently, an A-chain cDNA clone (D-1) was isolated from a transformed human glial cell cDNA library. We report the complete sequence of an A-chain cDNA clone (BT-1) isolated from a normal human umbilical vein endothelial (HUVE) cell cDNA library. BT-1 differs from the sequence of the D-1 clone by a 69 base pair deletion containing the predicted carboxy terminus of the protein. The mRNA levels of the A- and B-chains of PDGF in HUVE cells were analysed and shown to respond differently to the endothelial cell growth factor (ECGF).
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0028-0836 1476-4687
DOI:	10.1038/328619a0