new CMS source in Nicotiana developed via somatic cybridization between N. tabacum and N. alata

Cytoplasmic somatic hybrids (cybrids) between the two sexually incompatible species Nicotiana tabacum and Nicotiana alata were constructed. A total of 33 green regenerants were obtained after fusion of protoplasts from a tobacco cytoplasmic chlorophyll-deficient mutant and gamma irradiation-inactiva...

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Bibliographic Details
Published in:	Theoretical and applied genetics Vol. 97; no. 5/6; pp. 982 - 985
Main Authors:	Atanassov, I.I, Atanassova, S.A, Dragoeva, A.I, Atanassov, A.I
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer 01-10-1998 Berlin Springer Nature B.V
Subjects:	adenosinetriphosphatase atpa gene Biological and medical sciences chloroplast DNA Classical genetics, quantitative genetics, hybrids cybrids Cytogenetics Cytoplasm cytoplasmic inheritance cytoplasmic male sterility Fundamental and applied biological sciences. Psychology Genetic aspects genetic recombination Genetics of eukaryotes. Biological and molecular evolution genomics Helianthus hybrids interspecific hybridization mitochondrial DNA Nicotiana alata Nicotiana tabacum Physiological aspects Plant genetics plant morphology protoplast fusion Pteridophyta, spermatophyta restriction fragment length polymorphism somatic hybridization stamens structural genes Tobacco (Plant) Vegetals France Bulgaria Nucleocytoplasmic hybrid Dicotyledones Angiospermae Interspecific hybridization Stimulant plant Spermatophyta Mitochondrial DNA Solanaceae Nicotiana tabacum Cytoplasm Cytoplasmic inheritance Cytoplasmic male sterility
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Summary:	Cytoplasmic somatic hybrids (cybrids) between the two sexually incompatible species Nicotiana tabacum and Nicotiana alata were constructed. A total of 33 green regenerants were obtained after fusion of protoplasts from a tobacco cytoplasmic chlorophyll-deficient mutant and gamma irradiation-inactivated leaf protoplasts of N. alata. Twenty nine of them were male sterile and displayed an altered stamen morphology (formation of petaloid and stigmoid structures instead of stamens). Southern-blot analyses of eight CMS plants using N. alata-specific nuclear repetitive DNA and cpDNA probes revealed that they contained nuclear genetic material of N. tabacum and chloroplasts from N. alata. Restriction-enzyme analysis of mitochondrial DNAs of the cybrids in question showed different patterns consisting of an incomplete mix of mtDNA fragments from both parents, as well as new fragments. Southern-blot analysis of mtDNAs with a sunflower atpA probe gave the same recombinant hybridization pattern for all analyzed cybrids, indicating that high-frequency specific recombination occurs in the atpA region. Analysis of the progeny from three successive backcrosses of the studied cybrids with N. tabacum demonstrated a strict cytoplasmic inheritance of the male-sterile phenotype.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0040-5752 1432-2242
DOI:	10.1007/s001220050980