Portable fluorescence reader for Point-of-Care Molecular Diagnostic device

Polymerase Chain Reaction (PCR) is routinely used as a diagnostic tool for detection of genetic material of pathogens to confirm whether an individual is infected or not. The readout for quantifying PCR amplicon is still a bottleneck for development of field deployable portable system to perform PCR...

Full description

Saved in:

Bibliographic Details
Published in:	2019 IEEE 16th India Council International Conference (INDICON) pp. 1 - 4
Main Authors:	Srinivasan, Rajesh, Singh Rawat, Kapil, Aswathappa, Roopa, V. R., Eugene Christo, Siva Gorthi, Sai
Format:	Conference Proceeding
Language:	English
Published:	IEEE 01-12-2019
Subjects:	DNA Fluorescence Fluorescence readout Instruments Lenses Optical filters Optical variables measurement PCR Photodiodes Point of Care diagnostics
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Polymerase Chain Reaction (PCR) is routinely used as a diagnostic tool for detection of genetic material of pathogens to confirm whether an individual is infected or not. The readout for quantifying PCR amplicon is still a bottleneck for development of field deployable portable system to perform PCR tests in low-resource settings. Herein we present a portable fluorescence readout system for PCR amplicon readout with high accuracy and sensitivity that can detect as low as 18ng/μL of dsDNA, a concentration 10 times lower than those usually encountered in PCR amplicons. It utilizes a Single Board Computer (SBC) with integrated wireless connectivity, that enables a user friendly and robust system. An optimized system implementation assures a low (<; 1 watt) power consumption, enabling battery powered usage. We demonstrate the use and efficacy of the developed system as a proof of concept by performing a quantitative analysis of a PCR amplified functional gene belonging to Mycobacterium tuberculosis.
ISSN:	2325-9418
DOI:	10.1109/INDICON47234.2019.9030287