Search Results - "Assine Picchi, Gisele Fernanda"
-
1
Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4-deficient Trypanosoma cruzi cells
Published in Scientific reports (04-11-2021)“…Trypanosoma cruzi —the causative agent of Chagas disease—like other kinetoplastids, relies mostly on post-transcriptional mechanisms for regulation of gene…”
Get full text
Journal Article -
2
Knockout confirmation for hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture
Published in Memórias do Instituto Oswaldo Cruz (01-07-2014)“…Gene knockout is a widely used approach to evaluate loss-of-function phenotypes and it can be facilitated by the incorporation of a DNA cassette having a…”
Get full text
Journal Article -
3
Knockout of the CCCH zinc finger protein TcZC3H31 blocks Trypanosoma cruzi differentiation into the infective metacyclic form
Published in Molecular and biochemical parasitology (01-04-2018)“…T. cruzi knockout for the TcZC3H31 gene (TcZC3H31 KO) is incapable, both in vitro and in vivo, to differentiate into the metacyclic trypomastigote form…”
Get full text
Journal Article -
4
plastome sequence of the endemic Amazonian conifer, Retrophyllum piresii (Silba) C.N.Page, reveals different recombination events and plastome isoforms
Published in Tree genetics & genomes (01-02-2016)“…Retrophyllum piresii (Podocarpaceae) is an endemic conifer species from the Brazilian Amazonian Region, and very few data related to ecological and genetic…”
Get full text
Journal Article -
5
In cellulo and in vivo assays for compound testing against Trypanosoma cruzi
Published in STAR protocols (17-03-2023)“…Here, we describe a combined in cellulo and in vivo approach to identify compounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I…”
Get full text
Journal Article -
6
Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi
Published in STAR protocols (17-06-2022)“…This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We…”
Get full text
Journal Article -
7
Molecular characterization of type II topoisomerase in the endosymbiont-bearing Trypanosomatid Blastocrithidia culicis
Published in FEMS microbiology letters (01-04-2006)“…Abstract DNA topoisomerases are involved in DNA metabolism. These enzymes are inhibited by antimicrobial and antitumoral agents and might be important targets…”
Get full text
Journal Article -
8