Search Results - "Assine Picchi, Gisele Fernanda"

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    Knockout confirmation for hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture by Alcantara, Monica Visnieski, Fragoso, Stenio Perdigão, Picchi, Gisele Fernanda Assine

    Published in Memórias do Instituto Oswaldo Cruz (01-07-2014)
    “…Gene knockout is a widely used approach to evaluate loss-of-function phenotypes and it can be facilitated by the incorporation of a DNA cassette having a…”
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    Journal Article
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    In cellulo and in vivo assays for compound testing against Trypanosoma cruzi by Guerra-Slompo, Eloise Pavão, Picchi-Constante, Gisele Fernanda Assine, Marek, Martin, Romier, Christophe, Sippl, Wolfgang, Zanchin, Nilson Ivo Tonin

    Published in STAR protocols (17-03-2023)
    “…Here, we describe a combined in cellulo and in vivo approach to identify compounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I…”
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    Journal Article
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    Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi by Picchi-Constante, Gisele Fernanda Assine, Hiraiwa, Priscila Mazzocchi, Marek, Martin, Rogerio, Vanessa Zulkievicz, Guerra-Slompo, Eloise Pavão, Romier, Christophe, Zanchin, Nilson Ivo Tonin

    Published in STAR protocols (17-06-2022)
    “…This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We…”
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    Journal Article
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