Restin: a novel intermediate filament‐associated protein highly expressed in the Reed‐Sternberg cells of Hodgkin's disease

We have identified a cDNA coding for a protein of 160 kDa which is expressed in in vitro cultured human peripheral blood monocytes. The predicted amino acid sequence contains an alpha‐helical rod domain possessing features characteristic of intermediate filament proteins. However, the immunocytochem...

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Bibliographic Details
Published in:	The EMBO journal Vol. 11; no. 6; pp. 2103 - 2113
Main Authors:	Bilbe, G., Delabie, J., Brüggen, J., Richener, H., Asselbergs, F.A., Cerletti, N., Sorg, C., Odink, K., Tarcsay, L., Wiesendanger, W.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group 01-06-1992
Subjects:	Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cell Line Cells, Cultured CHO Cells Cloning, Molecular Cricetinae DNA, Neoplasm - genetics DNA, Neoplasm - isolation & purification Gene Library Hematologic and hematopoietic diseases Hodgkin Disease - genetics Hodgkin Disease - metabolism Humans Intermediate Filament Proteins - genetics Intermediate Filament Proteins - isolation & purification Intermediate Filament Proteins - metabolism Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Medical sciences Microtubule-Associated Proteins Molecular Sequence Data Monocytes - cytology Monocytes - metabolism Monocytes - pathology Neoplasm Proteins - genetics Neoplasm Proteins - isolation & purification Neoplasm Proteins - metabolism Protein Conformation Reed-Sternberg Cells - metabolism Sequence Homology, Nucleic Acid Transfection Vimentin - analysis Human Intermediary filament Monocyte Hodgkin disease Primary structure Malignant hemopathy Gene expression In vitro Proteins Complementary DNA Etiology Lymphoproliferative syndrome Molecular association Cytoskeleton
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Summary:	We have identified a cDNA coding for a protein of 160 kDa which is expressed in in vitro cultured human peripheral blood monocytes. The predicted amino acid sequence contains an alpha‐helical rod domain possessing features characteristic of intermediate filament proteins. However, the immunocytochemical staining pattern, abundance and solubility in Triton X‐100/high salt buffers suggest that this protein is probably only associated with the intermediate filament network and represents a new type of intermediate filament associated protein. In a survey of normal, inflammatory and human tumour tissue samples, this protein, which we have named restin, was found to be highly expressed in Reed‐Sternberg cells, the tumoral cells diagnostic for Hodgkin's disease. We suggest that restin overexpression may be a contributing factor in the progression of Hodgkin's disease.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0261-4189 1460-2075
DOI:	10.1002/j.1460-2075.1992.tb05269.x