Search Results - "Ash, David E."

Expression of peptidylarginine deiminase from Porphyromonas gingivalis in Escherichia coli: Enzyme purification and characterization by Rodríguez, Sofía B., Stitt, Barbara L., Ash, David E.

“…Porphyromonas gingivalis peptidylarginine deiminase (PAD) catalyzes the deimination of peptidylarginine residues of various peptides to produce…”
Get full text

Human Arginase II:  Crystal Structure and Physiological Role in Male and Female Sexual Arousal by Cama, Evis, Colleluori, Diana M, Emig, Frances A, Shin, Hyunshun, Kim, Soo Woong, Kim, Noel N, Traish, Abdulmaged M, Ash, David E, Christianson, David W

“…Arginase is a binuclear manganese metalloenzyme that catalyzes the hydrolysis of l-arginine to form l-ornithine and urea. The X-ray crystal structure of a…”
Get full text

Classical and Slow-Binding Inhibitors of Human Type II Arginase by Colleluori, Diana M, Ash, David E

“…Arginases catalyze the hydrolysis of l-arginine to yield l-ornithine and urea. Recent studies indicate that arginases, both the type I and type II isozymes,…”
Get full text

Inhibitor Coordination Interactions in the Binuclear Manganese Cluster of Arginase by Cama, Evis, Pethe, Stéphanie, Boucher, Jean-Luc, Han, Shoufa, Emig, Frances A, Ash, David E, Viola, Ronald E, Mansuy, Daniel, Christianson, David W

“…Arginase is a manganese metalloenzyme that catalyzes the hydrolysis of l-arginine to form l-ornithine and urea. The structure and stability of the binuclear…”
Get full text

Structure and function of arginases by Ash, David E

“…The arginases catalyze the divalent cation dependent hydrolysis of L-arginine to produce L-ornithine and urea. Although traditionally considered in terms of…”
Get more information

Structural and Functional Importance of First-Shell Metal Ligands in the Binuclear Manganese Cluster of Arginase I by Cama, Evis, Emig, Frances A, Ash, David E, Christianson, David W

“…Arginase is a binuclear manganese metalloenzyme that hydrolyzes l-arginine to form l-ornithine and urea. The three-dimensional structures of D128E, D128N,…”
Get full text

Structure of a unique binuclear manganese cluster in arginase by Kanyo, Zoltan F, Scolnick, Laura R, Ash, David E, Christianson, David W

Get full text

Slow and sustained nitric oxide releasing compounds inhibit multipotent vascular stem cell proliferation and differentiation without causing cell death by Curtis, Brandon M., Leix, Kyle Alexander, Ji, Yajing, Glaves, Richard Samuel Elliot, Ash, David E., Mohanty, Dillip K.

“…•Multipotent vascular stem cells (MVSCs) proliferate and differentiate.•Nitric oxide inhibits proliferation of MVSCs.•Nitric oxide inhibits MVSC…”
Get full text

Probing the role of the hyper-reactive histidine residue of arginase by Colleluori, Diana M., Reczkowski, Robert S., Emig, Frances A., Cama, Evis, Cox, J. David, Scolnick, Laura R., Compher, Kevin, Jude, Kevin, Han, Shoufa, Viola, Ronald E., Christianson, David W., Ash, David E.

“…Rat liver arginase (arginase I) is potently inactivated by diethyl pyrocarbonate, with a second-order rate constant of 113 M −1s −1 for the inactivation…”
Get full text

Dichotomous effects of isomeric secondary amines containing an aromatic nitrile and nitro group on human aortic smooth muscle cells via inhibition of cystathionine-γ-lyase by Ji, Yajing, Bowersock, Austin, Badour, Alec R., Vij, Neeraj, Juris, Stephen J., Ash, David E., Mohanty, Dillip K.

“…Excessive proliferation of vascular smooth muscle cells (SMC) is an important contributor to the progression of atherosclerosis. Inhibition of proliferation…”
Get full text

l-Arginine Binding to Liver Arginase Requires Proton Transfer to Gateway Residue His141 and Coordination of the Guanidinium Group to the Dimanganese(II,II) Center by Khangulov, Sergei V, Sossong, Thomas M, Ash, David E, Dismukes, G. Charles

“…Rat liver arginase contains a dimanganese(II,II) center per subunit that is required for catalytic hydrolysis of l-arginine to form urea and l-ornithine. A…”
Get full text

Purification of a Multipotent Antideath Activity from Bovine Liver and Its Identification as Arginase: Nitric Oxide-Independent Inhibition of Neuronal Apoptosis by Esch, Fred, Lin, Kuo-I, Hills, Anna, Zaman, Khalequz, Baraban, Jay M, Chatterjee, Sukalyan, Rubin, Lee, Ash, David E, Ratan, Rajiv R

“…Catalase is an antioxidant enzyme that has been shown to inhibit apoptotic or necrotic neuronal death induced by hydrogen peroxide. We report the purification…”
Get full text

Secondary amines containing one aromatic nitro group: Preparation, nitrosation, sustained nitric oxide release, and the synergistic effects of released nitric oxide and an arginase inhibitor on vascular smooth muscle cell proliferation by Curtis, Brandon, Payne, Thomas J., Ash, David E., Mohanty, Dillip K.

“…Secondary amines prepared from the reactions of linear aliphatic amines and 2,4-difluoronitrobenzene or 2,6-difluronitrobenzene at low temperatures were…”
Get full text

Altering the Binuclear Manganese Cluster of Arginase Diminishes Thermostability and Catalytic Function by Scolnick, Laura R, Kanyo, Zoltan F, Cavalli, R. Christopher, Ash, David E, Christianson, David W

“…Arginase is a thermostable (T m = 75 °C) binuclear manganese metalloenzyme which hydrolyzes l-arginine to form l-ornithine and urea. The three-dimensional…”
Get full text

EXAFS Comparison of the Dimanganese Core Structures of Manganese Catalase, Arginase, and Manganese-Substituted Ribonucleotide Reductase and Hemerythrin by Stemmler, Timothy L, Sossong, Thomas M, Goldstein, Jonathan I, Ash, David E, Elgren, Timothy E, Kurtz, Donald M, Penner-Hahn, James E

“…The solution structures of the binuclear Mn centers in arginase, Mn catalase, and the Mn-substituted forms of the Fe enzymes ribonucleotide reductase and…”
Get full text

Probing the Specificity Determinants of Amino Acid Recognition by Arginase by Shishova, Ekaterina Y, Di Costanzo, Luigi, Emig, Francis A, Ash, David E, Christianson, David W

“…Arginase is a binuclear manganese metalloenzyme that serves as a therapeutic target for the treatment of asthma, erectile dysfunction, and atherosclerosis. In…”
Get full text

Cysteine 351 is an essential nucleophile in catalysis by Porphyromonas gingivalis peptidylarginine deiminase by Rodríguez, Sofía B., Stitt, Barbara L., Ash, David E.

“…► Peptidylarginine deiminase from Porphyromonas gingivalis is inactivated by thiol-directed reagents. ► Cysteine 351 is the amino acid residue critical for…”
Get full text

Probing Erectile Function:  S-(2-Boronoethyl)-l-Cysteine Binds to Arginase as a Transition State Analogue and Enhances Smooth Muscle Relaxation in Human Penile Corpus Cavernosum by Kim, Noel N, Cox, J. David, Baggio, Ricky F, Emig, Frances A, Mistry, Sanjay K, Harper, Sandy L, Speicher, David W, Morris, Sidney M, Ash, David E, Traish, Abdulmaged, Christianson, David W

“…The boronic acid-based arginine analogue S-(2-boronoethyl)-l-cysteine (BEC) has been synthesized and assayed as a slow-binding competitive inhibitor of the…”
Get full text

Mutagenesis of Rat Liver Arginase Expressed in Escherichia coli: Role of Conserved Histidines by Cavalli, R. Christopher, Burke, Carl J, Soprano, Dianne Robert, Kawamoto, Susumu, Ash, David E

Get full text

Determination of mammalian arginase activity by Kepka-Lenhart, Diane, Ash, David E, Morris, Sidney M

“…Of all arginine catabolic enzymes, the arginases and nitric oxide (NO) synthases are the ones that are of greatest interest to many investigators. Mammalian…”
Get more information