Search Results - "Asbóth, B"

Mechanism of action of D-xylose isomerase by Asbóth, B, Náray-Szabó, G

“…The present knowledge on the stereochemical mechanism of action of glucose (or xylose) isomerase, one of the highest tonnage industrial enzymes, is summarized…”
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Specificity Assay of Serine Proteinases by Reverse-Phase High-Performance Liquid Chromatography Analysis of Competing Oligopeptide Substrate Library by Antal, József, Pál, Gábor, Asbóth, Bence, Buzás, Zsuzsanna, Patthy, András, Gráf, László

“…In this paper we present an HPLC method developed for quick activity and specificity analysis of serine proteinases. The method applies a carefully designed…”
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Remarkable Phylum Selectivity of a Schistocerca gregaria Trypsin Inhibitor: The Possible Role of Enzyme–Inhibitor Flexibility by Patthy, András, Amir, Sumaira, Malik, Zulfiquar, Bódi, Árpád, Kardos, József, Asbóth, Bence, Gráf, László

“…A 35-mer polypeptide isolated from the hemolymph of desert locust Schistocerca gregaria (SG) proved to be a canonical inhibitor of bovine trypsin (Ki = 0.2…”
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Crosslinking of glucoamylases via carbohydrates hardly affects catalysis but impairs stability by SASVARI, Z, ASBOTH, B

“…Mild oxidation of glucoamylases from Aspergillus niger (E.C.3.2.1.3.) with periodate, followed by incubation with adipic acid dihydrazide, covalently linked…”
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Colorado potato beetle larvae on potato plants expressing a locust proteinase inhibitor by Kutas, J, Kondrák, M, Szenthe, B, Patthy, A, Bánfalvi, Z, Nádasy, M, Gráf, L, Asbóth, B

“…The natural defence system of plants often involves inhibitors of digestive enzymes of their pests. Modem and environmental-friendly methods try to increase…”
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Transition-state stabilization at the oxyanion binding sites of serine and thiol proteinases: hydrolyses of thiono and oxygen esters by Asboth, Bence, Polgar, Laszlo

“…X-ray diffraction studies suggested that the tetrahedral intermediate formed during the catalysis by serine and thiol proteinases can be stabilized by hydrogen…”
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Cysteine proteases: The S2P2 hydrogen bond is more important for catalysis than is the analogous S1P1 bond by Asbo´th, B., Majer, Z., Polga´r, L.

“…High hydrophobicity of the second amino acid N‐terminal to the scissile bond (P2 residue) is generally considered to be the major factor in the specificity of…”
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The basic difference in catalyses by serine and cysteine proteinases resides in charge stabilization in the transition state by Polgár, L, Asbóth, B

“…Besides the mechanistic similarities, in particular acylenzyme formation, kinetic investigations and X-ray diffraction studies have revealed some differences…”
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Mechanism of action of cysteine proteinases: oxyanion binding site is not essential in the hydrolysis of specific substrates by Asboth, Bence, Stokum, Eva, Khan, Ikram U, Polgar, Laszlo

“…To study the possible stabilization of the oxyanion of the tetrahedral intermediate formed in the course of the catalyses by cysteine proteinases, papain,…”
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Disulfide bond formation between the active-site thiol and one of the several free thiol groups of chymopapain by Korodi, Ibolya, Asboth, Bence, Polgar, Laszlo

“…Chymopapain, a cysteine protease of papaya latex, has been purified with the use of fast protein liquid chromatography. Two homogeneous fractions were analyzed…”
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Subunit contact surface--an additional argument in favour of continuous folding during biosynthesis of proteins by Simon, I, Asbóth, B

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Substrate and inhibitor specificity of a transient, soluble proteolytic activity from sheep rumen by Baintner, K, Szabó, G C, Asbóth, B

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Is an amphiphilic region responsible for the haemolytic activity of Bacillus thuringiensis toxin? by Szabó, E, Murvai, J, Fábián, P, Fábián, F, Hollósi, M, Kajtár, J, Buzás, Z, Sajgó, M, Pongor, S, Asbóth, B

“…The amino acid sequence of the 27 kDa protein responsible for the haemolytic activity of Bacillus thuringiensis subsp. israelensis toxin has been analysed by…”
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On the stereochemistry of catalysis by serine proteases by Polgár, L, Asbóth, B

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On the enhanced catalytic activity of papain towards amide substrates by Asbóth, B, Polgár, L

“…According to the scanty literature data papain (EC. 3.4.4.10) reacts with ester and corresponding amide substrates at a similar rate (Glazer, Smith, 1971)…”
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Hydrolysis of alkyl ester and amide substrates by papain by Asbóth, B, Polgár, L

“…The ratio of the rate constants of acylation of papain with some amino acid ester and amide substrates is unexpectedly low. The contribution to this low ratio…”
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Stability of Arthrobacter D-xylose isomerase to denaturants and heat by RANGARAJAN, M, ASBOTH, B, HARTLEY, B. S

“…There was no inactivation of Mg(2+)-containing Arthrobacter D-xylose isomerase up to 1 h in 0-8 M-urea at 22 degrees C, but over this range there was rapid…”
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Structure determination and refinement of the Al3+ complex of the D254,256E mutant of Arthrobacter D-xylose isomerase at 2.40 A resolution. Further evidence for inhibitor-induced metal ion movement by Gérczei, T, Böcskei, Z, Szabó, E, Asbóth, B, Náray-Szabó, G

“…The structure of the D254.256E double mutant of Arthrobacter xylose isomerase with Al3+ at both metal-binding sites was determined by the molecular replacement…”
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Proteinase inhibitors from desert locust, Schistocerca gregaria: engineering of both P(1) and P(1)' residues converts a potent chymotrypsin inhibitor to a potent trypsin inhibitor by Malik, Z, Amir, S, Pál, G, Buzás, Z, Várallyay, E, Antal, J, Szilágyi, Z, Vékey, K, Asbóth, B, Patthy, A, Gráf, L

“…Two peptides, SGCI and SGTI, that inhibited chymotrypsin and trypsin, respectively, were isolated from the haemolymph of Schistocerca gregaria. Their primary…”
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Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato by KONDRAK, Mihaly, KUTAS, Janos, SZENTHE, Borbala, PATTHY, Andras, BANFALVI, Zsofia, NADASY, Miklos, GRAF, Laaszlo, ASBOTH, Bence

“…The cDNA for a 73-mer peptide containing two locust serine proteinase inhibitors was cloned, fused to the constitutive CaMV35S promoter and introduced into…”
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