Degradation of microcystins using immobilized microorganism isolated in an eutrophic lake

The final purpose of our series of studies is to establish a biological removal method of cyanobacteria and their toxic products using immobilized microorganisms that can lyse cyanobacteria and decompose microcystins. To establish the biological removal method in non-point areas and water purificati...

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Bibliographic Details
Published in:	Chemosphere (Oxford) Vol. 65; no. 1; pp. 117 - 124
Main Authors:	Tsuji, Kiyomi, Asakawa, Miki, Anzai, Yojiro, Sumino, Tatsuo, Harada, Ken-ichi
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-09-2006 Elsevier
Subjects:	Applied sciences Bacteria - classification Bacteria - growth & development Biodegradation Biodegradation, Environmental Biological and medical sciences Biological treatment of waters Bioreactors - microbiology Biotechnology Continental surface waters Cyanobacteria Cyanobacteria - growth & development Earth sciences Earth, ocean, space Engineering and environment geology. Geothermics Environment and pollution Eutrophication Exact sciences and technology Fresh Water - analysis Fresh Water - microbiology Freshwater Fundamental and applied biological sciences. Psychology Immobilization Industrial applications and implications. Economical aspects Microcystin Microcystins Natural water pollution Peptides, Cyclic - analysis Phylogeny Pollution Pollution, environment geology Sphingomonas Water Microbiology Water Pollutants - analysis Water treatment and pollution Asia Japan Japan, Honshu, Kanagawa Prefect., Tsukui L Biodegradation Microcystin Immobilization Cyanobacteria Microbial activity Cyclic peptides Identification Microcystis Eutrophication Toxin Decontamination Microbial origin Polypeptide Surface water Lakes Bacteria Water pollution Bioremediation Hepatotoxicity Entrapped microorganism Organic compounds
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Summary:	The final purpose of our series of studies is to establish a biological removal method of cyanobacteria and their toxic products using immobilized microorganisms that can lyse cyanobacteria and decompose microcystins. To establish the biological removal method in non-point areas and water purification plants, as the first step, we explored bacteria active against the cyanobacterial hepatotoxin microcystin in the present study. Eleven active bacteria were isolated from samples taken from Lakes Tsukui and Sagami, Japan. Among 3 strains (B-9 to B-11) with degradative activity, strain B-9 exhibited the strongest activity. The 16S rDNA sequence of the strain B-9 showed the highest similarity to that of Sphingomonas sp. Y2 (AB084247, 99% similarity). Microcystins-RR and -LR were completely degraded by strain B-9 (SC16) within 1 d, which led to an immobilized microorganism with a polyester resin. The degradation of microcystin-RR in a bioreactor using the immobilized strain B-9 was observed and microcystin-RR (>90%) was completely degraded after 24 h. Microcystin-RR was added to the lake water at regular intervals and the degradation after 24 h was observed in the bioreactor over a 72-d period. An over 80% removal efficiency continued for 2 months, showing that the life of the immobilized B-9 in terms of activity was at least 2 months under the optimized conditions. From these results, this immobilized B-9 is feasible for the practical treatment of microcystins in non-point areas and water purification plants.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0045-6535 1879-1298
DOI:	10.1016/j.chemosphere.2006.02.018