Niemann-Pick Disease, Type C1 Gene Expression in PBMCs is Associated with Interleukin 10 Serum Concentration: a Case-Control Study

Recent studies showed that atherosclerosis is a lysosomal storage disease (LSD) and Niemann-Pick disease type C1 (NPC1) is the most important protein of the lysosomal membrane that is involved in the removal of FC from lysosomes. Whereas several in vitro and in vivo studies have described the crosst...

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Bibliographic Details
Published in:	Clinical laboratory (Heidelberg) Vol. 64; no. 1; p. 17
Main Authors:	Zali, Fatemeh, Teimouri, Maryam, Shabani, Parisa, Vatannejad, Akram, Najafi, Mahdi, Shateri, Hossein, Asadnia, Mostafa, Doosti, Mahmood
Format:	Journal Article
Language:	English
Published:	Germany 01-01-2018
Subjects:	Aged Atherosclerosis - blood Atherosclerosis - genetics Carrier Proteins - genetics Case-Control Studies Cells, Cultured Gene Expression Humans Interleukin-10 - blood Leukocytes, Mononuclear - metabolism Lipids - blood Male Membrane Glycoproteins - genetics Middle Aged Niemann-Pick Disease, Type C - blood Niemann-Pick Disease, Type C - genetics Vitamin D - analogs & derivatives Vitamin D - blood
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Summary:	Recent studies showed that atherosclerosis is a lysosomal storage disease (LSD) and Niemann-Pick disease type C1 (NPC1) is the most important protein of the lysosomal membrane that is involved in the removal of FC from lysosomes. Whereas several in vitro and in vivo studies have described the crosstalk between lysosomal cholesterol accumulation and increased inflammation, there is no study addressing the correlation between NPC1 gene expression and an anti-inflammatory cytokine, interleukin 10 (IL-10) serum concentration in atherosclerotic patients. IL-10 and 25-hydroxyvitamin D serum concentrations were quantified by enzyme-linked immunosorbent assay (ELISA) in atherosclerotic patients (n = 40) and a control group (n = 40). NPC1 gene expression analysis was performed by quantitative real-time PCR, and correlation between the two parameters was assessed. Mean IL-10 serum concentration and peripheral blood mononuclear cells' (PBMCs) gene expression of NPC1, adjusted for drug consumption, age, and BMI, was not significantly different between the patient and control groups (p = 0.6 and 0.67 respectively). However, NPC1 gene expression showed positive significant correlation with IL-10 serum concentration (p = 0.04, r = 0.29). We also observed lower serum concentration of IL-10 in the subjects with lower 25-hydroxyvitamin D serum concentration (p = 0.034). Our findings supported the previous observations showing the contribution of lysosomal lipid homeostasis of PBMCs to inflammation and pathogenesis of atherosclerosis.
ISSN:	1433-6510
DOI:	10.7754/Clin.Lab.2017.170513