A Study of Gene Expression, Structure, and Contractility of iPSC-Derived Cardiac Myocytes from a Family with Heart Disease due to LMNA Mutation

Genetic mutations to the Lamin A/C gene ( LMNA ) can cause heart disease, but the mechanisms making cardiac tissues uniquely vulnerable to the mutations remain largely unknown. Further, patients with LMNA mutations have highly variable presentation of heart disease progression and type. In vitro pat...

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Bibliographic Details
Published in:	Annals of biomedical engineering Vol. 49; no. 12; pp. 3524 - 3539
Main Authors:	Mehrabi, Mehrsa, Morris, Tessa A., Cang, Zixuan, Nguyen, Cecilia H. H., Sha, Yutong, Asad, Mira N., Khachikyan, Nyree, Greene, Taylor L., Becker, Danielle M., Nie, Qing, Zaragoza, Michael V., Grosberg, Anna
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-12-2021 Springer Nature B.V
Subjects:	Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Biophysics C gene Cardiomyocytes Cardiovascular disease Cardiovascular diseases Classical Mechanics Coronary artery disease Gene expression Gene sequencing Genes Heart Heart diseases Heterogeneity Muscle contraction Mutation Myocytes Original Original Article Patients Pluripotency Stem cells Structure-function relationships Disease presentation iPSC-derived cardiomyocyte Dysmorphic nuclei Lamin A/C mutation
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Summary:	Genetic mutations to the Lamin A/C gene ( LMNA ) can cause heart disease, but the mechanisms making cardiac tissues uniquely vulnerable to the mutations remain largely unknown. Further, patients with LMNA mutations have highly variable presentation of heart disease progression and type. In vitro patient-specific experiments could provide a powerful platform for studying this phenomenon, but the use of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) introduces heterogeneity in maturity and function thus complicating the interpretation of the results of any single experiment. We hypothesized that integrating single cell RNA sequencing (scRNA-seq) with analysis of the tissue architecture and contractile function would elucidate some of the probable mechanisms. To test this, we investigated five iPSC-CM lines, three controls and two patients with a (c.357-2A>G) mutation. The patient iPSC-CM tissues had significantly weaker stress generation potential than control iPSC-CM tissues demonstrating the viability of our in vitro approach. Through scRNA-seq, differentially expressed genes between control and patient lines were identified. Some of these genes, linked to quantitative structural and functional changes, were cardiac specific, explaining the targeted nature of the disease progression seen in patients. The results of this work demonstrate the utility of combining in vitro tools in exploring heart disease mechanics.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Associate Editor Stefan M Duma oversaw the review of this article.
ISSN:	0090-6964 1573-9686
DOI:	10.1007/s10439-021-02850-8