Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol

Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch culture on ethanol

After analysis of batch culture and identification of the ways for prolongation of citric acid active synthesis by yeast, repeat-batch (RB) cultivation was suggested. Yarrowia lipolytica strain RB cultivation was studied and optimal conditions for cultivation selected. It was shown that when applyin...

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Bibliographic Details
Published in:Applied microbiology and biotechnology Vol. 53; no. 5; pp. 525 - 529
Main Authors: Arzumanov, T.E, Shishkanova, N.V, Finogenova, T.V
Format: Journal Article
Language:English
Published: Germany Springer Nature B.V 01-05-2000
Subjects:
Acids
Biosynthesis
citric acid
Citric Acid - metabolism
Comparative analysis
Cultivation
Culture Media
Enzymatic activity
enzyme activity
Enzymes
Ethanol
Ethanol - metabolism
oxidation
Saccharomycetales - growth & development
Saccharomycetales - metabolism
Yarrowia lipolytica
Yeasts
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Summary:After analysis of batch culture and identification of the ways for prolongation of citric acid active synthesis by yeast, repeat-batch (RB) cultivation was suggested. Yarrowia lipolytica strain RB cultivation was studied and optimal conditions for cultivation selected. It was shown that when applying RB cultivation, better results were obtained than for batch cultivation. The activity of the culture remained stable after cultivation for more than 700 h. Comparative analysis of enzyme activities confirmed the regularity of the effect described, as the activity of practically of all the enzymes participating in ethanol oxidation and citric acid biosynthesis remained stable over time during RB cultivation. Advantages of RB cultivation for the production of citric acid by yeast are discussed.
Bibliography:http://dx.doi.org/10.1007/s002530051651
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ISSN:0175-7598
1432-0614
DOI:10.1007/s002530051651