The effects of metyrosine on ischemia-reperfusion-induced oxidative ovarian injury in rats: Biochemical and histopathological assessment

The effects of metyrosine on ischemia-reperfusion-induced oxidative ovarian injury in rats: Biochemical and histopathological assessment

The aim of this study is to investigate the effect of metyrosine on ischemia-reperfusion (I/R) induced ovarian injury in rats in terms of biochemistry and histopathology. Rats were divided into: ovarian I/R (OIR), ovarian I/R+50 mg/kg metyrosine (OIRM) and sham (SG) operations. OIRM group received 5...

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Published in:Anais da Academia Brasileira de Ciências Vol. 95; no. 2; p. e20201586
Main Authors: Yapca, Omer E, Yildiz, Gulsah A, Mammadov, Renad, Kurt, Nezahat, Gundogdu, Betul, Arslan, Yusuf K, Suleyman, Halis, Cetin, Nihal
Format: Journal Article
Language:English
Published: Brazil Academia Brasileira de Ciências 01-01-2023
Subjects:
alpha-Methyltyrosine - metabolism
alpha-Methyltyrosine - pharmacology
Animals
cyclo-oxygenase enzyme
Cyclooxygenase 2 - metabolism
Cyclooxygenase 2 - pharmacology
Female
Glutathione
Ischemia - metabolism
Ischemia - pathology
ischemia-reperfusion
metyrosine
MULTIDISCIPLINARY SCIENCES
ovarian tissues
Ovary - metabolism
Oxidative Stress
rat
Rats
Rats, Wistar
Reperfusion
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Superoxide Dismutase - metabolism
cyclo-oxygenase enzyme
ischemia-reperfusion
ovarian tissues
rat
metyrosine
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Summary:The aim of this study is to investigate the effect of metyrosine on ischemia-reperfusion (I/R) induced ovarian injury in rats in terms of biochemistry and histopathology. Rats were divided into: ovarian I/R (OIR), ovarian I/R+50 mg/kg metyrosine (OIRM) and sham (SG) operations. OIRM group received 50 mg/kg metyrosine one hour before the application of the anesthetic agent, OIR and SG group rats received equal amount of distilled water to be used as a solvent orally through cannula. Following the application of the anesthetic agent, ovaries of OIRM and OIR group rats were subjected to ischemia and reperfusion, each of which took two hours. This biochemical experiment findings revealed high levels of malondialdehyde (MDA) and cyclo-oxygenase-2 (COX-2) and low levels of total glutathione (tGSH), superoxide dismutase (SOD) and cyclo-oxygenase-1 (COX-1) in the ovarian tissue of OIR group, with significant histopathological injury. In metyrosine group, MDA and COX-2 levels were lower than the OIR group whereas tGSH, SOD and COX-1 levels were higher, with slighter histopathological injury. Our experimental findings indicate that metyrosine inhibits oxidative and pro-inflammatory damage associated with ovarian I/R in rats. These findings suggest that metyrosine could be useful in the treatment of ovarian injury associated with I/R.
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content type line 23
ISSN:0001-3765
1678-2690
1678-2690
DOI:10.1590/0001-3765202320201586