Evaluating the Feasibility of Five Candidate DNA Barcoding Loci for Philippine Lasianthus Jack ( Lasiantheae : Rubiaceae )

Evaluating the Feasibility of Five Candidate DNA Barcoding Loci for Philippine Lasianthus Jack ( Lasiantheae : Rubiaceae )

The pantropical genus Jack is identified for high phenotypic plasticity making traditional taxonomic identification difficult. Having some members with important medicinal properties, a precise complimentary identification through DNA barcoding is needed for species delineation. In this study, 12 sa...

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Bibliographic Details
Published in:Pharmacognosy Magazine Vol. 13; no. 52; pp. 553 - 558
Main Authors: Arshed, Muhammad Jefte C, Valdez, Jr, Marcos B, Alejandro, Grecebio Jonathan D
Format: Journal Article
Language:English
Published: India Medknow Publications and Media Pvt. Ltd 01-10-2017
Sage Publications Ltd
Medknow Publications & Media Pvt Ltd
Subjects:
Biological research
Chloroplasts
Deoxyribonucleic acid
DNA
DNA barcoding
Evaluation
Genetic aspects
Identification
Identification and classification
Morphology
Original
Phylogenetics
Plant taxonomy
Polymerase chain reaction
Quantitative trait loci
Rubiaceae
Studies
Philippines
United States > US
Germany
Lasianthus
nrDNA
cpDNA
Philippines
DNA barcoding
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Summary:The pantropical genus Jack is identified for high phenotypic plasticity making traditional taxonomic identification difficult. Having some members with important medicinal properties, a precise complimentary identification through DNA barcoding is needed for species delineation. In this study, 12 samples representing six Philippine species were used to determine the most efficient barcoding loci among the cpDNA markers ( K, L, 16, and T-F) and nrDNA (ITS) based on the criteria of universality, discriminatory power, and resolution of species. The results revealed that ITS has the recommended primer universality, greatest interspecific divergences, and average resolution of species. Among the cpDNA markers, K and L are recommended but with minimal resolution of species. While T-F showed moderate interspecific variations and resolution of species, 16 has the lowest interspecific divergence and resolution of species. Consequently, ITS is the potential ideal DNA barcode for species. ITS, K, and 16 markers have the excellent amplification and sequence qualityITS marker has the highest interspecific divergence with the maximum values, followed by K, L, T-F, and 16, respectivelyAll markers except 16 yielded average resolution to Lasianthus speciesITS marker is the most ideal locus in terms of excellent universality, high interspecific discriminatory ability, and average species resolution. ITS: Internal Transcribe Spacer, K: maturase K, L: ribulose-1,5-biphospahte-carboxylase, 16: ribosomal protein 16 small subunit gene.
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ISSN:0973-1296
0976-4062
DOI:10.4103/pm.pm_1_17