Search Results - "Arpino, James A J"

Crystal structure of enhanced green fluorescent protein to 1.35 Å resolution reveals alternative conformations for Glu222 by Arpino, James A J, Rizkallah, Pierre J, Jones, D Dafydd

“…Enhanced Green Fluorescent Protein (EGFP) is one of the most widely used engineered variants of the original wild-type Green Fluorescent Protein. Here, we…”
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In-frame amber stop codon replacement mutagenesis for the directed evolution of proteins containing non-canonical amino acids: identification of residues open to bio-orthogonal modification by Arpino, James A J, Baldwin, Amy J, McGarrity, Adam R, Tippmann, Eric M, Jones, D Dafydd

“…Expanded genetic code approaches are a powerful means to add new and useful chemistry to proteins at defined residues positions. One such use is the…”
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Structural Basis for Efficient Chromophore Communication and Energy Transfer in a Constructed Didomain Protein Scaffold by Arpino, James A. J, Czapinska, Honorata, Piasecka, Anna, Edwards, Wayne R, Barker, Paul, Gajda, Michal J, Bochtler, Matthias, Jones, D. Dafydd

“…The construction of useful functional biomolecular components not currently part of the natural repertoire is central to synthetic biology. A new…”
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A Modular Method for Directing Protein Self-Assembly by Arpino, James A. J, Polizzi, Karen Marie

“…Proteins are versatile macromolecules with diverse structure, charge, and function. They are ideal building blocks for biomaterials for drug delivery,…”
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Revealing the complexity of ionic liquid–protein interactions through a multi-technique investigation by Bui-Le, Liem, Clarke, Coby J., Bröhl, Andreas, Brogan, Alex P. S., Arpino, James A. J., Polizzi, Karen M., Hallett, Jason P.

“…Ionic liquids offer exciting possibilities for biocatalysis as solvent properties provide rare opportunities for customizable, energy-efficient bioprocessing…”
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Random Single Amino Acid Deletion Sampling Unveils Structural Tolerance and the Benefits of Helical Registry Shift on GFP Folding and Structure by Arpino, James A.J., Reddington, Samuel C., Halliwell, Lisa M., Rizkallah, Pierre J., Jones, D. Dafydd

“…Altering a protein’s backbone through amino acid deletion is a common evolutionary mutational mechanism, but is generally ignored during protein engineering…”
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Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein by Arpino, James A. J., Rizkallah, Pierre J., Jones, D. Dafydd

“…Single‐amino‐acid deletions are a common part of the natural evolutionary landscape but are rarely sampled during protein engineering owing to limited and…”
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Tuning the dials of Synthetic Biology by Arpino, James A J, Hancock, Edward J, Anderson, James, Barahona, Mauricio, Stan, Guy-Bart V, Papachristodoulou, Antonis, Polizzi, Karen

“…Synthetic Biology is the 'Engineering of Biology' - it aims to use a forward-engineering design cycle based on specifications, modelling, analysis,…”
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Simplified mechanistic models of gene regulation for analysis and design by Hancock, Edward J., Stan, Guy-Bart, Arpino, James A. J., Papachristodoulou, Antonis

“…Simplified mechanistic models of gene regulation are fundamental to systems biology and essential for synthetic biology. However, conventional simplified…”
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Expanded chemical diversity sampling through whole protein evolution by Baldwin, Amy J, Arpino, James A J, Edwards, Wayne R, Tippmann, Eric M, Jones, D Dafydd

“…A directed evolution method has been developed that allows random substitution of a contiguous trinucleotide sequence for TAG throughout a target gene for use…”
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Transposon-based approaches for generating novel molecular diversity during directed evolution by Jones, D Dafydd, Arpino, James A J, Baldwin, Amy J, Edmundson, Matthew C

“…This chapter introduces a set of transposon-based methods that were developed to sample trinucleotide deletion, trinucleotide replacement, and domain…”
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