Effects of gamma rays on the immunogenicity (IgG types) of ovalbumin
Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with gamma rays, yielding toxoids with good immunogenicity. However, little is known about...
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| Published in: | Radiation physics and chemistry (Oxford, England : 1993) Vol. 71; no. 1; pp. 483 - 485 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Elsevier Ltd
01-09-2004
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with gamma rays, yielding toxoids with good immunogenicity. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we used ovalbumin as a model to investigate possible immunogenic differences between native and irradiated proteins.
Native ovalbumin (2
mg/ml in 150
mM NaCl) was irradiated with 2
kGy of
60Co gamma rays with a 570
Gy/h dose rate. B10.PL mice (
n=5) were then immunized with either the native or the irradiated protein. After three immunizations, serum samples were collected and the antibody titers and isotypes were determined by enzyme-linked immunoadsorbant assay. Our data indicate that no difference could be noticed when the antibody titers of the two groups were compared. However, the isotyping assays indicates that the native protein induced high levels of IgG1, while its irradiated counterpart displayed mostly IgG2b antibodies. These data suggest that after irradiation, an antigen known to induce a Th2 response, is able to switch the immune system towards a Th1 pattern. |
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| ISSN: | 0969-806X 1879-0895 |
| DOI: | 10.1016/j.radphyschem.2004.06.004 |