Search Results - "Arndt, Katja M"

Photocontrol of Coiled-Coil Proteins in Living Cells by Zhang, Fuzhong, Timm, Katharina A., Arndt, Katja M., Woolley, G. Andrew

“…Light switching of the activity of a coiled‐coil protein, the AP‐1 transcription factor, in living cells was made possible by the introduction of a designed…”
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Strategies for the photo-control of endogenous protein activity by Brechun, Katherine E, Arndt, Katja M, Woolley, G Andrew

“…•Optogenetic effectors allow roles of endogenous proteins to be probed.•Effector caging strategies rely on photo-controlled conformational changes.•Effectors…”
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A user-friendly, low-cost turbidostat with versatile growth rate estimation based on an extended Kalman filter by Hoffmann, Stefan A, Wohltat, Christian, Müller, Kristian M, Arndt, Katja M

“…For various experimental applications, microbial cultures at defined, constant densities are highly advantageous over simple batch cultures. Due to high costs,…”
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Coiled Coil Domains: Stability, Specificity, and Biological Implications by Mason, Jody M, Arndt, Katja M

“…Twist and shout: Coiled‐coil forming α‐helices are of great significance in understanding tertiary structural formation (the figure shows GCN4; an example of a…”
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Strategies for Enzymatic Inactivation of the Veterinary Antibiotic Florfenicol by Müller, Marik M, Nedielkov, Ruslan, Arndt, Katja M

“…Large quantities of the antibiotic florfenicol are used in animal farming and aquaculture, contaminating the ecosystem with antibiotic residues and promoting…”
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rAAV Engineering for Capsid-Protein Enzyme Insertions and Mosaicism Reveals Resilience to Mutational, Structural and Thermal Perturbations by Feiner, Rebecca C, Teschner, Julian, Teschner, Kathrin E, Radukic, Marco T, Baumann, Tobias, Hagen, Sven, Hannappel, Yvonne, Biere, Niklas, Anselmetti, Dario, Arndt, Katja M, Müller, Kristian M

“…Recombinant adeno-associated viruses (rAAV) provide outstanding options for customization and superior capabilities for gene therapy. To access their full…”
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Semirational Design of Jun-Fos Coiled Coils with Increased Affinity: Universal Implications for Leucine Zipper Prediction and Design by Mason, Jody M., Schmitz, Mark A., Müller, Kristian M., Arndt, Katja M.

“…Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. For this reason, nine homologues of the AP-1 leucine zipper region…”
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Improving coiled coil stability while maintaining specificity by a bacterial hitchhiker selection system by Kükenshöner, Tim, Wohlwend, Daniel, Niemöller, Christoph, Dondapati, Padmarupa, Speck, Janina, Adeniran, Adebola V., Nieth, Anita, Gerhardt, Stefan, Einsle, Oliver, Müller, Kristian M., Arndt, Katja M.

“…The design and selection of peptides targeting cellular proteins is challenging and often yields candidates with undesired properties. Therefore we deployed a…”
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Controlling leucine-zipper partner recognition in cells through modification of a-g interactions by Azuma, Yusuke, Kükenshöner, Tim, Ma, Guangyong, Yasunaga, Jun-ichiro, Imanishi, Miki, Tanaka, Gen, Nakase, Ikuhiko, Maruno, Takahiro, Kobayashi, Yuji, Arndt, Katja M, Matsuoka, Masao, Futaki, Shiroh

“…By focusing on the a-g interactions, successful design and selection were accomplished to obtain a leucine-zipper segment that discriminates the appropriate…”
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Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V by Baumann, Tobias, Arndt, Katja M, Müller, Kristian M

“…DNA fragments carrying internal recognition sites for the restriction endonucleases intended for cloning into a target plasmid pose a challenge for…”
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An in vivo library-versus-library selection of optimized protein-protein interactions by Michnick, Stephen W, Pelletier, Joelle N, Arndt, Katja M, Plückthun, Andreas

“…We describe a rapid and efficient in vivo library-versus-library screening strategy for identifying optimally interacting pairs of heterodimerizing…”
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Factors Influencing the Dimer to Monomer Transition of an Antibody Single-Chain Fv Fragment by Arndt, Katja M, Müller, Kristian M, Plückthun, Andreas

“…Antibody single-chain Fv (scFv) fragments are able to form dimers under certain conditions, and the extent of dimerization appears to depend on linker length,…”
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iPEP: peptides designed and selected for interfering with protein interaction and function by Mason, Jody M, M Müller, Kristian, Arndt, Katja M

“…Semi-rational design is combined with PCAs (protein-fragment complementation assays) and phage-display screening techniques to generate a range of iPEPs…”
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Modification of 3D-Printed PLA Structures Using Photo-Iniferter Polymerization: Toward on-Demand Antimicrobial Water Filters by Lehnen, Anne-Catherine, Hanke, Sebastian, Schneider, Matthias, Radelof, Charlotte M L, Perestrelo, Joana, Reinicke, Stefan, Reifarth, Martin, Taubert, Andreas, Arndt, Katja M, Hartlieb, Matthias

“…Water filtration is an important application field to ensure the accessibility of clean drinking water. As requirements and contaminants vary on a local level,…”
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Long-range transcriptional interference in E. coli used to construct a dual positive selection system for genetic switches by Hoffmann, Stefan A, Kruse, Sabrina M, Arndt, Katja M

“…We have investigated transcriptional interference between convergent genes in E. coli and demonstrate substantial interference for inter-promoter distances of…”
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Selection of Protein–Protein Interactions of Desired Affinities with a Bandpass Circuit by Brechun, Katherine E., Arndt, Katja M., Woolley, G. Andrew

“…We have developed a genetic circuit in Escherichia coli that can be used to select for protein–protein interactions of different strengths by changing…”
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Detection of Incorporation of p‑Coumaric Acid into Photoactive Yellow Protein Variants in Vivo by Brechun, Katherine E, Zhen, Danlin, Jaikaran, Anna, Borisenko, Vitali, Kumauchi, Masato, Hoff, Wouter D, Arndt, Katja M, Woolley, G. Andrew

“…We report the design and characterization of photoactive yellow protein (PYP)–blue fluorescent protein (mTagBFP) fusion constructs that permit the direct assay…”
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Characterizing Transcriptional Interference between Converging Genes in Bacteria by Hoffmann, Stefan A, Hao, Nan, Shearwin, Keith E, Arndt, Katja M

“…Antisense transcription is common in naturally occurring genomes and is increasingly being used in synthetic genetic circuitry as a tool for gene expression…”
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Comparison of In Vivo Selection and Rational Design of Heterodimeric Coiled Coils by Arndt, Katja M., Pelletier, Joelle N., Müller, Kristian M., Plückthun, Andreas, Alber, Tom

“…To investigate how electrostatic interactions restrict the associations of coiled coils, we improved a heterodimeric coiled coil (WinZip-A1B1) by in vivo…”
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Modular adeno-associated virus (rAAV) vectors used for cellular virus-directed enzyme prodrug therapy by Hagen, Sven, Baumann, Tobias, Wagner, Hanna J., Morath, Volker, Kaufmann, Beate, Fischer, Adrian, Bergmann, Stefan, Schindler, Patrick, Arndt, Katja M., Müller, Kristian M.

“…The pre-clinical and clinical development of viral vehicles for gene transfer increased in recent years and a recombinant adeno-associated virus (rAAV) drug…”
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