Immunity Regulatory DNAs Share Common Organizational Features in Drosophila

Infection results in the rapid activation of immunity genes in the Drosophila fat body. Two classes of transcription factors have been implicated in this process: the REL-containing proteins, Dorsal, Dif, and Relish, and the GATA factor Serpent. Here we present evidence that REL-GATA synergy plays a...

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Bibliographic Details
Published in:	Molecular cell Vol. 13; no. 1; pp. 19 - 32
Main Authors:	Senger, Kate, Armstrong, Grant W, Rowell, William J, Kwan, Jennifer M, Markstein, Michele, Levine, Michael
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 16-01-2004
Subjects:	Animals Animals, Genetically Modified Binding Sites Cells, Cultured Consensus Sequence Dif protein Dorsal protein Drosophila Drosophila - genetics Drosophila - immunology Drosophila - microbiology Drosophila Proteins - metabolism Embryo, Nonmammalian Enhancer Elements, Genetic Fat Body - cytology Fat Body - immunology Fat Body - metabolism Gene Expression Regulation Genes, Insect Immunity - genetics Larva Models, Genetic Regulatory Sequences, Nucleic Acid Relish protein Sequence Deletion Serpent protein Transcription Factors - metabolism Transcriptional Activation
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Summary:	Infection results in the rapid activation of immunity genes in the Drosophila fat body. Two classes of transcription factors have been implicated in this process: the REL-containing proteins, Dorsal, Dif, and Relish, and the GATA factor Serpent. Here we present evidence that REL-GATA synergy plays a pervasive role in the immune response. SELEX assays identified consensus binding sites that permitted the characterization of several immunity regulatory DNAs. The distribution of REL and GATA sites within these DNAs suggests that most or all fat-specific immunity genes contain a common organization of regulatory elements: closely linked REL and GATA binding sites positioned in the same orientation and located near the transcription start site. Aspects of this “regulatory code” are essential for the immune response. These results suggest that immunity regulatory DNAs contain constrained organizational features, which may be a general property of eukaryotic enhancers.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1097-2765 1097-4164
DOI:	10.1016/S1097-2765(03)00500-8