A T cell receptor specific for an HLA-A03:01-restricted epitope in the endogenous retrovirus ERV-K-Env exhibits limited recognition of its cognate epitope

A T cell receptor specific for an HLA-A03:01-restricted epitope in the endogenous retrovirus ERV-K-Env exhibits limited recognition of its cognate epitope

Transposable elements (TEs) are often expressed at higher levels in tumor cells than normal cells, implicating these genomic regions as an untapped pool of tumor-associated antigens. In ovarian cancer (OC), protein from the TE ERV-K is frequently expressed by tumor cells. Here we determined whether...

Full description

Saved in:
Bibliographic Details
Published in:Mobile DNA Vol. 15; no. 1; pp. 19 - 18
Main Authors: Grundy, Erin E, Shaw, Lauren C, Wang, Loretta, Lee, Abigail V, Argueta, James Castro, Powell, Jr, Daniel J, Ostrowski, Mario, Jones, R Brad, Cruz, C Russell Y, Gordish-Dressman, Heather, Chappell, Nicole P, Bollard, Catherine M, Chiappinelli, Katherine B
Format: Journal Article
Language:English
Published: England BioMed Central Ltd 09-10-2024
BioMed Central
BMC
Subjects:
Antigens
Care and treatment
Diagnosis
Endogenous retroviruses
Health aspects
Immunotherapy
Ovarian cancer
T cell receptor
T cells
Transposable elements
Transposons
Tumor immunology
United States
Endogenous retroviruses
T cell receptor
Immunotherapy
Transposable elements
Tumor immunology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transposable elements (TEs) are often expressed at higher levels in tumor cells than normal cells, implicating these genomic regions as an untapped pool of tumor-associated antigens. In ovarian cancer (OC), protein from the TE ERV-K is frequently expressed by tumor cells. Here we determined whether the targeting of previously identified epitope in the envelope gene (env) of ERV-K resulted in target antigen specificity against cancer cells. We found that transducing healthy donor T cells with an ERV-K-Env-specific T cell receptor construct resulted in antigen specificity only when co-cultured with HLA-A*03:01 B lymphoblastoid cells. Furthermore, in vitro priming of several healthy donors with this epitope of ERV-K-Env did not result in target antigen specificity. These data suggest that the T cell receptor is a poor candidate for targeting this specific ERV-K-Env epitope and has limited potential as a T cell therapy for OC.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1759-8753
1759-8753
DOI:10.1186/s13100-024-00333-w