CCL28 modulates neutrophil responses during infection with mucosal pathogens

The chemokine CCL28 is highly expressed in mucosal tissues, but its role during infection is not well understood. Here we show that CCL28 promotes neutrophil accumulation in the gut of mice infected with and in the lung of mice infected with . Neutrophils isolated from the infected mucosa expressed...

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Published in:	eLife Vol. 13
Main Authors:	Walker, Gregory T, Perez-Lopez, Araceli, Silva, Steven, Lee, Michael H, Bjånes, Elisabet, Dillon, Nicholas, Brandt, Stephanie L, Gerner, Romana R, Melchior, Karine, Norton, Grant J, Argueta, Felix A, Dela Pena, Frenchesca, Park, Lauren, Sosa-Hernandez, Victor A, Cervantes-Diaz, Rodrigo, Romero-Ramirez, Sandra, Cartelle Gestal, Monica, Maravillas-Montero, Jose L, Nuccio, Sean-Paul, Nizet, Victor, Raffatellu, Manuela
Format:	Journal Article
Language:	English
Published:	England eLife Sciences Publications Ltd 28-08-2024 eLife Sciences Publications, Ltd
Subjects:	Acinetobacter Antimicrobial activity Antimicrobial agents Asthma Bone marrow Bronchopulmonary infection Cell activation Cell surface chemokine Chemokines Experiments Histopathology Immune clearance Immune response Immunology and Inflammation Infections Inflammatory bowel disease Leukocytes (neutrophilic) Lymphatic system Microbiology and Infectious Disease mucosa Mucosal immunity neutrophil Neutrophils Pathogens Phagocytosis Reactive oxygen species Salmonella Spleen Statistical analysis Tumor necrosis factor-TNF microbiology mouse infectious disease inflammation human immunology
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Summary:	The chemokine CCL28 is highly expressed in mucosal tissues, but its role during infection is not well understood. Here we show that CCL28 promotes neutrophil accumulation in the gut of mice infected with and in the lung of mice infected with . Neutrophils isolated from the infected mucosa expressed the CCL28 receptors CCR3 and, to a lesser extent, CCR10, on their surface. The functional consequences of CCL28 deficiency varied between the two infections: mice were highly susceptible to gut infection but highly resistant to otherwise lethal lung infection. , unstimulated neutrophils harbored pre-formed intracellular CCR3 that was rapidly mobilized to the cell surface following phagocytosis or inflammatory stimuli. Moreover, CCL28 stimulation enhanced neutrophil antimicrobial activity, production of reactive oxygen species, and formation of extracellular traps, all processes largely dependent on CCR3. Consistent with the different outcomes in the two infection models, neutrophil stimulation with CCL28 boosted the killing of but not . CCL28 thus plays a critical role in the immune response to mucosal pathogens by increasing neutrophil accumulation and activation, which can enhance pathogen clearance but also exacerbate disease depending on the mucosal site and the infectious agent.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work.
ISSN:	2050-084X 2050-084X
DOI:	10.7554/eLife.78206