Loop-Mediated Isothermal Amplification for the Diagnosis of Sporotrichosis by Sporothrix brasiliensis

We aimed to develop and validate a Loop-mediated Isothermal Amplification (LAMP) assay to Sporothrix brasiliensis . LAMP reaction was developed using six primers designed based on calmodulin gene. In the LAMP reaction, we tested twenty isolates of S. brasiliensis from animals and humans, along with...

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Bibliographic Details
Published in:	Mycopathologia (1975) Vol. 189; no. 5; p. 73
Main Authors:	Araujo, Marcella Donadel, Poester, Vanice Rodrigues, Schirmer, Helena, Mattevi, Vanessa Suñe, Trápaga, Mariana Rodrigues, Xavier, Melissa Orzechowski, Teixeira, Marcus de Melo, Almeida-Paes, Rodrigo, Zancopé-Oliveira, Rosely Maria, Severo, Cecília Bittencourt
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer Netherlands 01-10-2024 Springer Nature B.V
Subjects:	Animal models Animals Biomedical and Life Sciences Calmodulin Calmodulin - genetics Deoxyribonucleic acid Diagnosis Disease Models, Animal DNA DNA Primers - genetics Eukaryotic Microbiology Gene amplification Genetic testing Humans Life Sciences Medical Microbiology Mice Microbial Ecology Microbiology Molecular Diagnostic Techniques - methods Nucleic Acid Amplification Techniques - methods Plant Sciences Sensitivity analysis Sensitivity and Specificity Short Communication Sporothrix - classification Sporothrix - genetics Sporothrix - isolation & purification Sporothrix brasiliensis Sporotrichosis Sporotrichosis - diagnosis Sporotrichosis - microbiology Sporotrichosis - veterinary spp LAMP Zoonosis Molecular assay Sporothrix spp
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Summary:	We aimed to develop and validate a Loop-mediated Isothermal Amplification (LAMP) assay to Sporothrix brasiliensis . LAMP reaction was developed using six primers designed based on calmodulin gene. In the LAMP reaction, we tested twenty isolates of S. brasiliensis from animals and humans, along with ten tissue samples extracted from the left footpad of mice that had been experimentally infected with S. brasiliensis . In addition, it included DNA samples from various other fungal species for specificity evaluation. All S. brasiliensis isolates yielded positive results in the LAMP, and the limit of DNA detection was 1 ng/μL. All murine samples were positive in the test while DNA from other fungal species were all negative, resulting in 100% of sensitivity and specificity of primers. LAMP diagnosis technique is a promising alternative to sporotrichosis diagnosis, in a simple and cost-effective way. Further studies are warranted to validate this technique using animal model samples obtained from both humans and animals.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Undefined-3
ISSN:	0301-486X 1573-0832 1573-0832
DOI:	10.1007/s11046-024-00880-z