Antimicrobial and antibiofilm action of Casbane Diterpene from Croton nepetaefolius against oral bacteria

Antimicrobial and antibiofilm action of Casbane Diterpene from Croton nepetaefolius against oral bacteria

Abstract Objective The antibacterial activity of Casbane Diterpene (CD) was evaluated in vitro against Streptococcus oralis , S. mutans , S. salivarius , S. sobrinus , S. mitis and S. sanguinis . The viability of planktonic cells was analysed by susceptibility tests (MIC and MBC) and antibiofilm act...

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Published in:Archives of oral biology Vol. 57; no. 5; pp. 550 - 555
Main Authors: Sá, Nairley Cardoso, Cavalcante, Theodora Thays Arruda, Araújo, Amanda Ximenes, Santos, Hélcio Silva dos, Albuquerque, Maria Rose Jane Ribeiro, Bandeira, Paulo Nogueira, Cunha, Rodrigo Maranguape Silva da, Cavada, Benildo Sousa, Teixeira, Edson Holanda
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-05-2012
Subjects:
Advanced Basic Science
Analysis of Variance
Anti-Infective Agents - chemistry
Anti-Infective Agents - pharmacology
Biofilm
Biofilms - drug effects
Casbane Diterpene
Colony Count, Microbial
Croton
Dentistry
Diterpenes - chemistry
Diterpenes - pharmacology
Gentian Violet
Humans
Microbial Sensitivity Tests
Microbial Viability - drug effects
Molecular Structure
Oral bacteria
Plant Extracts - chemistry
Plant Extracts - pharmacology
Streptococcus
Streptococcus - drug effects
Streptococcus oralis
Streptococcus salivarius
Biofilm
Casbane Diterpene
Oral bacteria
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Summary:Abstract Objective The antibacterial activity of Casbane Diterpene (CD) was evaluated in vitro against Streptococcus oralis , S. mutans , S. salivarius , S. sobrinus , S. mitis and S. sanguinis . The viability of planktonic cells was analysed by susceptibility tests (MIC and MBC) and antibiofilm action was assayed. Methods The minimal inhibitory and bactericidal concentrations (MIC and MBC) of oral Streptococcus were evaluated through microdilution tests. To assay antibiofilm activity, biofilms were generated on 96-wells polystyrene plates under the presence of CD and quantified by a crystal violet technique and colonies forming units counting. Results The CD isolated from Croton nepetaefolius showed antimicrobial effect on planktonic forms and biofilms of oral pathogens, with MIC values of 62.5 μg/mL against Streptococcus oralis and values between 125 and 500 μg/mL against S. mutans , S. salivarius , S. sobrinus , S. mitis and S. sanguinis . CD showed an inhibitory effect on S. mutans biofilm formation at 250 μg/mL, and a decrease on viable cell of 94.28% compared to the normal biofilm growth. Conclusions The compound CD can be considered as a promising molecule for the treatment against oral pathogens responsible for dental biofilm.
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ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2011.10.016