Genetic mapping of Uba3 O.2.2 , a pupal lethal mutation in Drosophila melanogaster

An EMS mutagenesis screen was conducted in Drosophila melanogaster to identify growth control mutants. The multi-institution Fly-CURE consortium phenotypically characterized the O.2.2 mutant using the FLP/FRT system which displayed a mutant lethal phenotype with reduced head development, and darkene...

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Published in:microPublication biology Vol. 2022
Main Authors: Mast, Elizabeth, Bieser, Kayla L, Abraham-Villa, Mary, Adams, Vanessa, Akinlehin, Akinwonuola J, Aquino, Lynarose Z, Austin, Joseph L, Austin, Abigail K, Beckham, Carissa N, Bengson, Ethan J, Bieszk, Amanda, Bogard, Brianna L, Brennan, Rowan C, Brnot, Rebecca M, Cirone, Nicholas J, Clark, Mason R, Cooper, Brianna N, Cruz, Dennys, Daprizio, Katlyn A, DeBoe, Jason, Dencker, Michaela M, Donnelly, Laura L, Driscoll, Leanne, DuBeau, Ryan J, Durso, Sirada W, Ejub, Adam, Elgosbi, Waad, Estrada, Melanie, Evins, Kaeli, Fox, Pearl D, France, Jacob M, Franco Hernandez, Maira G, Garcia, Lizbeth A, Garl, Olivia, Gorsuch, Myeerah R, Gorzeman-Mohr, Mikayla A, Grothouse, Madison E, Gubbels, Megan E, Hakemiamjad, Romina, Harvey, Chloé V, Hoeppner, Madeline A, Ivanov, Jessica L, Johnson, Veronica M, Johnson, Jessica L, Johnson, Ashton, Johnston, Kaleigh, Keller, Katie R, Kennedy, Breanna T, Killian, Levi R, Klumb, Marissa, Koehn, Olivia L, Koym, Aaron S, Kress, Kari J, Landis, Regan E, Lewis, Kaitlyn N, Lim, Enosh, Lopez, Ilcen K, Lowe, D'Artagnan, Luengo Carretero, Paula, Lunaburg, Grace, Mallinder, Samantha L, Marshall, Natalie A, Mathew, Jessica, Mathew, Jasmine, Mcmanaway, Hailee S, Meegan, Emily N, Meyst, Jacob D, Miller, Meredith J, Minogue, Colin K, Mohr, Alina A, Moran, Cristhian I, Moran, Adrian, Morris, Morgan D, Morrison, Michael D, Moses, Emmily A, Mullins, Cade J, Neri, Citlalli I, Nichols, Jess M, Nickels, Breanna R, Okai, Akosua M, Okonmah, Chiedu, Paramo, Makena, Paramo, Meagan, Parker, Sydney L, Parmar, Neil K, Paschal, Jacob, Patel, Prem, Patel, Deep, Perkins, Erica B, Perry, Madelyn M, Perry, Zachary, Pollock, Amanda A, Portalatin, Oxxyris, Proffitt, Kamron S, Queen, Jason T, Quemeneur, Alexis C, Richardson, Amelia G, Rosenberger, Kaylee, Rutherford, Allison M, Santos-Perez, Itchel X
Format: Journal Article
Language:English
Published: United States Caltech Library 2022
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Summary:An EMS mutagenesis screen was conducted in Drosophila melanogaster to identify growth control mutants. The multi-institution Fly-CURE consortium phenotypically characterized the O.2.2 mutant using the FLP/FRT system which displayed a mutant lethal phenotype with reduced head development, and darkened ocular tissue. Complementation mapping was conducted to identify the affected gene. A failure to complement was identified in Uba3 , resulting in the identification of the novel allele, Uba3 O.2.2 . Uba3 is a known disruptor of the cell cycle and our data are consistent with early larval/embryonic lethality displayed in numerous species.
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ISSN:2578-9430
DOI:10.17912/micropub.biology.000542