Antitrypanosomal effects of petroleum ether, chloroform and methanol extracts of Artemisia maciverae Linn

Petroleum ether, chloroform and methanol extracts of A. maciverae were studied in vitro and in vivo for activity against Trypanosoma brucei brucei in Swiss albino mice. Thereafter, the chloroform extract which showed the highest activity in both in vitro and in vivo assessments was subjected to bioa...

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Bibliographic Details
Published in:	Indian journal of experimental biology Vol. 47; no. 12; pp. 981 - 986
Main Authors:	Ene, A C, Atawodi, S E, Ameh, D A, Nnamani, C N, Apeh, Y E O
Format:	Journal Article
Language:	English
Published:	India 01-12-2009
Subjects:	Alkaloids - analysis Alkanes Animals Artemisia - chemistry Chloroform Methanol Mice Parasitemia - drug therapy Parasitemia - parasitology Plant Extracts - chemistry Plant Extracts - pharmacology Solvents Triterpenes - analysis Trypanocidal Agents - chemistry Trypanocidal Agents - pharmacology Trypanosoma brucei brucei - drug effects Trypanosoma brucei brucei - pathogenicity
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Summary:	Petroleum ether, chloroform and methanol extracts of A. maciverae were studied in vitro and in vivo for activity against Trypanosoma brucei brucei in Swiss albino mice. Thereafter, the chloroform extract which showed the highest activity in both in vitro and in vivo assessments was subjected to bioassay-guided fractionation. The crude extracts and the fractions of the chloroform extract of A. maciverae were screened for phytochemicals and secondary metabolites. Combined fractions 54-57 of this extract showed the highest in vitro antitrypanosomal activity, and at 10 mg/kg body weight, this fraction cleared the parasitemia completely from T. brucei brucei infected Swiss albino mice after 7 days of treatment. There was no statistically significant difference in the level of parasitemia when the infected mice treated with this fraction was compared with the standard trypanocidal drug, diminal. The results of the phytochemical analysis showed that the crude extracts contained secondary metabolites like flavonoids, triterpenes, terpenoids, tannins, phlobatannins and alkaloids, while the active fraction contains only triterpenes and alkaloids. It can be inferred that fraction 54-57 contains the active component responsible for the high antitrypanosomal activity of the chloroform extract of A. maciverae.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0019-5189