Detection by tissue printing of stone fruit viroids, from Europe, the Mediterranean and North and South America

Detection by tissue printing of stone fruit viroids, from Europe, the Mediterranean and North and South America

The two viroids known to affect stone fruit trees are Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd). Viroids can be detected by biological, biochemical or molecular methods. The two first approaches are time consuming and expensive, with the limiting step for molecular techniques be...

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Bibliographic Details
Published in:Acta horticulturae no. 657; pp. 379 - 383
Main Authors: Torres, H, Gómez, G, Pallás, V, Stamo, B, Shalaby, A, Aouane, B, Gavriel, I, Kominek, P, Caglayan, K, Sipahioglu, M, Michelutti, R, Myrta, A, Fiore, N
Format: Journal Article
Language:English
Published: International Society for Horticultural Science 01-01-2004
Subjects:
apricots
bioprinting
fruit trees
Hop stunt viroid
Peach latent mosaic viroid
peaches
plums
screening
viroids
Czech Republic
Cyprus
Turkey (country)
Egypt
Algeria
Chile
Albania
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Summary:The two viroids known to affect stone fruit trees are Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd). Viroids can be detected by biological, biochemical or molecular methods. The two first approaches are time consuming and expensive, with the limiting step for molecular techniques being the sample preparation process. In the present study, direct tissue-printing on membranes, a procedure that avoids sample extraction, has been applied on a large scale for an initial screening of PLMVd and HSVd in different regions from Europe (Albania and the Czech Republic), the Mediterranean basin (Algeria, Cyprus, Egypt and Turkey), and North- (Canada) and South-America (Chile). PLMVd was detected in peach trees from all countries surveyed except Egypt, with incidence ranging from 52% (Albania) to 5.4% (Algeria). HSVd was detected in peaches, apricots and plums, in Albania, Egypt and Turkey. The incidence of HSVd in the West part of Turkey both in apricot (66%) and peach (57%) was particularly high.
Bibliography:http://www.actahort.org/books/657/657_60.htm
ISSN:0567-7572
2406-6168
DOI:10.17660/ActaHortic.2004.657.60