Amplifiation of the c-MYC gene in acinar prostate adenocarcinoma. Morphogenic comparisons

The purpose of this work was to evaluate gene amplification in the substrate of prostate acinar adenocarcinoma at various Gleason scores and various stages of the disease, taking into account the morphological characteristics of the tumor. The number of cases in the study was 82, including the contr...

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Bibliographic Details
Published in:	Arkhiv patologii Vol. 86; no. 3; p. 30
Main Authors:	Khorzhevskii, V A, Alymova, E V, Kirichenko, A K, Gappoev, S V, Anzhiganova, Yu V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 2024
Subjects:	Aged Carcinoma, Acinar Cell - genetics Carcinoma, Acinar Cell - pathology Gene Amplification Genes, myc - genetics Humans Male Middle Aged Prostatic Neoplasms - genetics Prostatic Neoplasms - pathology Proto-Oncogene Proteins c-myc - genetics perineural invasion c-MYC gene amplification acinar adenocarcinoma of the prostate lymphovascular invasion
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Summary:	The purpose of this work was to evaluate gene amplification in the substrate of prostate acinar adenocarcinoma at various Gleason scores and various stages of the disease, taking into account the morphological characteristics of the tumor. The number of cases in the study was 82, including the control group - 12 cases. Morphological assessment included: determination of the total Gleason score, grading group, assessment of lymphovascular/perineural invasion, and architectural characteristics of the tumor. Gene amplification was assessed by FISH using the c-MYC (8q24)/SE8 probe. In all cases of the study group, amplification of the c-MYC gene was detected in the tumor, with a significant difference from the control group ( <0.05). When assessing cases with 4-6 fold copies of the gene, significant differences were established between patients with stages II and III of the disease and stage IV (10.0 and 13.5 versus 30.0) ( <0.05). Cluster amplification of the c-MYC gene was detected with equal frequency in groups of patients with stages III and IV of the disease, while in stage II of the disease, the event almost did not occur ( <0.05). A significant increase in the level of c-MYC gene amplification was found in groups with advanced stages of the disease ( <0.02). Non-cluster amplification significantly distinguishes T4M0 and T4M1 stage patients from the rest with a significant increase in the score ( <0.05). In the metastatic stage of the disease, there was an increase c-MYC gene amplification compared to the non-metastatic stage ( <0.02). The copy number of the c-MYC gene was significantly higher in cases with perineural and lymphovascular invasion, as well as in cases of cribriform tumor organization ( <0.05). Amplification of the gene in prostate tumor cells is associated with advanced stages of the disease (T4M0 and T4M1) with an increase in the copy number of the gene during the metastatic stage of the process. It was found that increased amplification of the gene distinguishes groups of patients whose tumors exhibit perineural and lymphovascular invasion, as well as a cribriform pattern of tumor organization.
ISSN:	0004-1955
DOI:	10.17116/patol20248603130