The Osteoblast Transcriptome in Developing Zebrafish Reveals Key Roles for Extracellular Matrix Proteins Col10a1a and Fbln1 in Skeletal Development and Homeostasis

Zebrafish are now widely used to study skeletal development and bone-related diseases. To that end, understanding osteoblast differentiation and function, the expression of essential transcription factors, signaling molecules, and extracellular matrix proteins is crucial. We isolated Sp7-expressing...

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Bibliographic Details
Published in:	Biomolecules (Basel, Switzerland) Vol. 14; no. 2; p. 139
Main Authors:	Raman, Ratish, Antony, Mishal, Nivelle, Renaud, Lavergne, Arnaud, Zappia, Jérémie, Guerrero-Limón, Gustavo, Caetano da Silva, Caroline, Kumari, Priyanka, Sojan, Jerry Maria, Degueldre, Christian, Bahri, Mohamed Ali, Ostertag, Agnes, Collet, Corinne, Cohen-Solal, Martine, Plenevaux, Alain, Henrotin, Yves, Renn, Jörg, Muller, Marc
Format:	Journal Article Web Resource
Language:	English
Published:	Switzerland MDPI AG 23-01-2024 Multidisciplinary Digital Publishing Institute (MDPI)
Subjects:	Analysis Animals Arthritis Biochemistry, biophysics & molecular biology Biochimie, biophysique & biologie moléculaire Bone matrix Bones Cartilage Cell Differentiation col10a1a Collagen Type X Collagen Type X - genetics Collagen Type X - physiology ECM Extracellular Matrix Extracellular Matrix - genetics Extracellular Matrix Proteins Extracellular Matrix Proteins - genetics Extracellular Matrix Proteins - metabolism fbln1 FGF8 Fibroblast growth factor 8 fibulin Gene expression Gene regulation Genes Genetic aspects Homeostasis Homeostasis - genetics Larvae Life sciences Matrix protein Mineralization Minerals Minerals - metabolism Mutants Mutation Osteoarthritis osteoblast Osteoblastogenesis Osteoblasts Osteoblasts - metabolism Physiological aspects Proteins Protocol Sciences du vivant skeletal development Skeleton Subpopulations Transcription factors transcriptome Transcriptome - genetics Transcriptomes Transcriptomics vertebra Vertebrae Zebra fish Zebrafish Zebrafish - genetics Zebrafish - growth & development Belgium Netherlands FGF8 transcriptome vertebra fbln1 osteoblast zebrafish skeletal development ECM gene expression col10a1a
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Summary:	Zebrafish are now widely used to study skeletal development and bone-related diseases. To that end, understanding osteoblast differentiation and function, the expression of essential transcription factors, signaling molecules, and extracellular matrix proteins is crucial. We isolated Sp7-expressing osteoblasts from 4-day-old larvae using a fluorescent reporter. We identified two distinct subpopulations and characterized their specific transcriptome as well as their structural, regulatory, and signaling profile. Based on their differential expression in these subpopulations, we generated mutants for the extracellular matrix protein genes and to study their functions. The mutant larvae display reduced chondrocranium size and decreased bone mineralization, while in adults a reduced vertebral thickness and tissue mineral density, and fusion of the caudal fin vertebrae were observed. In contrast, mutants showed an increased mineralization of cranial elements and a reduced ceratohyal angle in larvae, while in adults a significantly increased vertebral centra thickness, length, volume, surface area, and tissue mineral density was observed. In addition, absence of the opercle specifically on the right side was observed. Transcriptomic analysis reveals up-regulation of genes involved in collagen biosynthesis and down-regulation of Fgf8 signaling in mutants. Taken together, our results highlight the importance of bone extracellular matrix protein genes and in skeletal development and homeostasis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 info:eu-repo/grantAgreement/EC/H2020/766347 scopus-id:2-s2.0-85185901729 EU MSCA-ITN project
ISSN:	2218-273X 2218-273X
DOI:	10.3390/biom14020139