Engineering of the membrane of fibroblast cells with virus-specific antibodies: A novel biosensor tool for virus detection

A novel concept for the assay of viral antigens is described. The methodological approach is based on a membrane-engineering process involving the electroinsertion of virus-specific antibodies in the membranes of fibroblast cells. As a representative example, Vero fibroblasts were engineered with an...

Full description

Saved in:

Bibliographic Details
Published in:	Biosensors & bioelectronics Vol. 24; no. 4; pp. 1027 - 1030
Main Authors:	Moschopoulou, Georgia, Vitsa, Katerina, Bem, Frederic, Vassilakos, Nikos, Perdikaris, Antonis, Blouhos, Petros, Yialouris, Costas, Frosyniotis, Dimitris, Anthopoulos, Ioannis, Mangana, Olga, Nomikou, Kiki, Rodeva, Velichka, Kostova, Dimitrina, Grozeva, Stanislava, Michaelides, Alexandros, Simonian, Alex, Kintzios, Spiridon
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 01-12-2008
Subjects:	Animals Antibodies, Viral - genetics Antibodies, Viral - immunology Bioelectric recognition assay Biological Assay - instrumentation Biological Assay - methods Biosensing Techniques - instrumentation Biosensing Techniques - methods Cell biosensor Cell Membrane - physiology Cell Membrane - virology Cercopithecus aethiops Cucumber green mottle mosaic virus Cucumber mosaic virus Cucumovirus - genetics Cucumovirus - immunology Cucumovirus - isolation & purification Cytomegalovirus Electrochemistry - instrumentation Electrochemistry - methods Equipment Design Equipment Failure Analysis Genetic Engineering - methods Membrane-engineering Reproducibility of Results Sensitivity and Specificity Tobacco rattle virus Vero Cells Cucumber green mottle mosaic virus Bioelectric recognition assay Membrane-engineering Tobacco rattle virus Cell biosensor
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A novel concept for the assay of viral antigens is described. The methodological approach is based on a membrane-engineering process involving the electroinsertion of virus-specific antibodies in the membranes of fibroblast cells. As a representative example, Vero fibroblasts were engineered with antibodies against Cucumber mosaic virus (CMV) and used for the construction of an ultra-sensitive miniature cell biosensor system. The attachment of a homologous virus triggered specific changes to the cell membrane potential that were measured by appropriate microelectrodes, according to the principle of the bioelectric recognition assay (BERA). No change in the membrane potential was observed upon cell contact with the heterologous cucumber green mottle mosaic virus (CGMMV). Fluorescence microscopy observations showed that attachment of CMV particles to membrane-engineered cells was associated with membrane hyperpolarization and increased [Ca 2+] cyt. In an additional field-based application, we were able to detect CMV-infected tobacco plants at an essentially 100% level of accuracy.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0956-5663 1873-4235
DOI:	10.1016/j.bios.2008.06.039