Dietary Self-Selection Can Compensate an Age-Related Decrease of Rat Liver 20 S Proteasome Activity Observed With Standard Diet

Aged Lou female rats (33 months) submitted to a self-selection regimen showed a decrease in protein intake (down to 11% of the total intake), whereas mature rats (18 months) selected a high percentage of protein (20% of the total intake) similar to the protein content of the standard diet. To find o...

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Published in:	The journals of gerontology. Series A, Biological sciences and medical sciences Vol. 53A; no. 3; pp. B173 - B179
Main Authors:	Anselmi, Brigitte, Conconi, Mariangela, Veyrat-Durebex, Christelle, Turlin, Evelyne, Biville, Francis, Alliot, Josette, Friguet, Bertrand
Format:	Journal Article
Language:	English
Published:	United States The Gerontological Society of America 01-05-1998 Gerontological Society of America Oxford University Press
Subjects:	Aging - metabolism Animals Cellular biology Chromatography, Ion Exchange Cysteine Endopeptidases - isolation & purification Cysteine Endopeptidases - metabolism Diet Dietary Proteins Electrophoresis, Polyacrylamide Gel Enzymes Feeding Behavior - physiology Female Food Preferences - physiology Liver Liver - enzymology Liver - growth & development Multienzyme Complexes - isolation & purification Multienzyme Complexes - metabolism Proteasome Endopeptidase Complex Proteins Rats Rats, Inbred Strains Rodents
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Summary:	Aged Lou female rats (33 months) submitted to a self-selection regimen showed a decrease in protein intake (down to 11% of the total intake), whereas mature rats (18 months) selected a high percentage of protein (20% of the total intake) similar to the protein content of the standard diet. To find out if this decrease in protein intake would prevent an observed age-related decrease in proteasome activity, four peptidase activities and oxidized protein degradation were tested with proteasome purified from the liver of 18- and 33-month-old rats. The peptidylglutamyl-pepHde hydrolase activity, which is decreased with age for rats fed the standard diet, was restored in the self-selecting old rats to the level observed for the mature rats. Degradation of oxidized glutamine synthetase, which is also decreased with age for rats fed the standard diet, was partly restored. Proteasome from self-selecting old rats showed a slight increase in trypsin-like and chymotrypsin-like activities as compared to proteasome from old rats fed the standard diet. Two-dimensional gel electrophoresis followed by quantitative analysis of the pattern of proteasome subunits revealed an increase in the intensity of two protein spots for proteasome from old rats fed the standard diet as compared with proteasome from either mature rats or self-selecting old rats. These findings may have important implications in aging for proteasome-mediated proteolysis and subsequent accumulation of oxidatively damaged protein.
Bibliography:	ark:/67375/HXZ-P00XJ70X-M istex:3C10A388D6AF5FD0EC937461CE7E2818AB972362 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1079-5006 1758-535X
DOI:	10.1093/gerona/53A.3.B173