Renal vascular reactivity to vasopressin in rats with diabetes mellitus

We evaluated how renal vascular reactivity to vasopressin changes when nitric oxide (NO) synthesis varies, as has been reported to occur in the course of insulin-dependent diabetes mellitus. Renal vasoconstrictor responses to vasopressin were obtained in young and older Sprague–Dawley control rats (...

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Published in:	European journal of pharmacology Vol. 431; no. 3; pp. 321 - 329
Main Authors:	Loichot, Cécile, Anjuère, Jérôme, Nisato, Dino, De Jong, Wybren, Imbs, Jean-Louis, Barthelmebs, Mariette
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 23-11-2001 Elsevier
Subjects:	Age Aging Animals Associated diseases and complications Biological and medical sciences Blood Pressure - drug effects Diabetes Diabetes Mellitus, Experimental - physiopathology Diabetes Mellitus, Type 2 - physiopathology Diabetes. Impaired glucose tolerance Dose-Response Relationship, Drug Endocrine pancreas. Apud cells (diseases) Endocrinopathies Fundamental and applied biological sciences. Psychology Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis In Vitro Techniques Injections, Intravenous Kidney - drug effects Kidney - physiopathology Male Medical sciences Nitric oxide (NO) Organ Size Rats Rats, Sprague-Dawley Regional Blood Flow - drug effects Renal vascular reactivity Time Factors Vasoconstriction Vasopressin Vasopressins - administration & dosage Vasopressins - pharmacology Vertebrates: endocrinology Diabetes Vasopressin Renal vascular reactivity Age Nitric oxide (NO) Endocrinopathy Rat Peptide hormone Neuropeptide Kidney Neurohypophyseal hormone Complication Kidney disease Regional blood flow Urinary system disease Pathophysiology Diabetes mellitus Rodentia Normal In vitro Biological activity Young animal In vivo Vertebrata Mammalia Urinary system Animal Nitric oxide Aged animal Interindividual comparison
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Summary:	We evaluated how renal vascular reactivity to vasopressin changes when nitric oxide (NO) synthesis varies, as has been reported to occur in the course of insulin-dependent diabetes mellitus. Renal vasoconstrictor responses to vasopressin were obtained in young and older Sprague–Dawley control rats (3 and 10 months old) and in age-matched diabetic rats that had been treated with streptozotocin (60 mg/kg i.v.) at the age of 2 months. In young rats, vasopressin (3–1000 ng/kg/min i.v.) induced in vivo a dose-dependent decrease in renal blood flow, which was diminished in streptozotocin diabetic rats ( P<0.05). Similarly, in in vitro perfused kidneys, the concentration–response curve for vasopressin (0.03–10 nM) was shifted 3-fold to the right in kidneys isolated from young diabetic rats ( P<0.05). This shift was abolished in the presence of an inhibitor of nitric oxide synthesis, N G-nitro- l-arginine (100 μM), in the perfusate. In 10-month-old rats, the in vivo renal vasoconstrictor dose–response curve to vasopressin was shifted 10-fold to the left as compared to that for young rats ( P<0.001). This shift was similar in both control and diabetic rats. In conclusion, the present study documented the existence of hyporesponsiveness to vasopressin in the early stage of diabetes, possibly related to nitric oxide overproduction. In contrast, renal vascular hyperreactivity to vasopressin occurs with aging, whether the rats are diabetic or not.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-2999 1879-0712
DOI:	10.1016/S0014-2999(01)01404-2