Evaluation of antioxidant activity of muscle and skin protein hydrolysates from giant kingfish, Caranx ignobilis (Forsskål, 1775)

Summary To assess the antioxidant properties of fish protein hydrolysate from giant kingfish Caranx ignobilis (Forsskål, 1775), muscle and skin were obtained using various proteases (papain, pepsin, trypsin and α‐chymotrypsin), respectively. Antioxidant activities of the resulting hydrolysates were...

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Published in:	International journal of food science & technology Vol. 47; no. 2; pp. 274 - 281
Main Authors:	Nazeer, R. A., Anila Kulandai, K.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-02-2012 Wiley-Blackwell
Subjects:	Amino acids Antioxidant Antioxidants Biological and medical sciences Caranx ignobilis essential amino acids fish protein hydrolysate Food industries Free radicals Fundamental and applied biological sciences. Psychology Hydrolysates Muscles Papain Proteins Scavenging Evaluation essential amino acids Hydrolysate fish protein hydrolysate Muscle Skin Antioxidant Protein Caranx ignobilis
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Summary:	Summary To assess the antioxidant properties of fish protein hydrolysate from giant kingfish Caranx ignobilis (Forsskål, 1775), muscle and skin were obtained using various proteases (papain, pepsin, trypsin and α‐chymotrypsin), respectively. Antioxidant activities of the resulting hydrolysates were evaluated using 2, 2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity, reducing power and metal chelating activity. Among the hydrolysates, peptic of muscle (37 ± 1.0; 0.290 ± 0.012; 63.30 ± 0.57) and tryptic of skin (36.2 ± 1.0; 0.190 ± 0.110; 63 ± 0.57) hydrolysates, which had the highest free radical scavenging activity, was subjected to purification using Sephadex G‐25. The fractionated hydrolysates were superior to the original hydrolysates in the antioxidative activity tested. The active fractions (MF2 and SF2) effectively inhibited lipid peroxidation in linoleic acid emulsion system, and the activity was compared with α‐tocopherol. The amino acid profile of MF2 and SF2 showed a high level of essential amino acids, and the most abundant amino acids were in the order His (12.01% and 7.08%), Phe (7.05% and 6.18%) and Lys (6.76% and 4.74%). Conclusively, C. ignobilis muscle and skin hydrolysates could be a promising rich source of natural antioxidants.
Bibliography:	ArticleID:IJFS2836 istex:6F74C0659419F58D92375E92A23BF5C33B56127A ark:/67375/WNG-TJ6SZCFK-F ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0950-5423 1365-2621
DOI:	10.1111/j.1365-2621.2011.02836.x