Search Results - "Angela Proctor"

"Fix and assay": separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative by Proctor, Angela, Allbritton, Nancy L

“…Chemical cytometry using capillary electrophoresis (CE) is a powerful tool for measuring single-cell enzyme activity. However, these measurements are often…”
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Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells by Wang, Yuli, DiSalvo, Matthew, Gunasekara, Dulan B, Dutton, Johanna, Proctor, Angela, Lebhar, Michael S, Williamson, Ian A, Speer, Jennifer, Howard, Riley L, Smiddy, Nicole M, Bultman, Scott J, Sims, Christopher E, Magness, Scott T, Allbritton, Nancy L

“…Background & Aims Three-dimensional organoid culture has fundamentally changed the in vitro study of intestinal biology enabling novel assays; however, its use…”
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Chemical fixation to arrest phospholipid signaling for chemical cytometry by Proctor, Angela, Sims, Christopher E., Allbritton, Nancy L.

“…•High quality separation of phosphatidylinositol isomers was developed.•Exogenously added fluorescent phospholipid is retained in cells after…”
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Measurement of Protein Kinase B Activity in Single Primary Human Pancreatic Cancer Cells by Proctor, Angela, Herrera-Loeza, S. Gabriela, Wang, Qunzhao, Lawrence, David S., Yeh, Jen Jen, Allbritton, Nancy L.

“…An optimized peptide substrate was used to measure protein kinase B (PKB) activity in single cells. The peptide substrate was introduced into single cells, and…”
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Fluorous enzymatic synthesis of phosphatidylinositides by Huang, Weigang, Proctor, Angela, Sims, Christopher E, Allbritton, Nancy L, Zhang, Qisheng

“…A fluorous tagging strategy coupled with enzymatic synthesis is introduced to efficiently synthesize multiple phosphatidylinositides, which are then directly…”
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The Early Promise of TBRI Implementation in Schools by Reid, Mark J, Proctor, Angela M, Brooks, Thomas R

“…The program known as Trust Based Relational Intervention® (TBRI®) began as an exploration into the detrimental behaviors of foster and adopted children placed…”
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Selection and optimization of enzyme reporters for chemical cytometry by Proctor, Angela, Wang, Qunzhao, Lawrence, David S, Allbritton, Nancy L

“…Chemical cytometry, sensitive analytical measurements of single cells, reveals inherent heterogeneity of cells within a population which is masked or averaged…”
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Required hydrophobicity of fluorescent reporters for phosphatidylinositol family of lipid enzymes by Waybright, Jarod, Huang, Weigang, Proctor, Angela, Wang, Xiaoyang, Allbritton, Nancy L., Zhang, Qisheng

“…The phosphatidylinositol (PtdIns) family of lipids plays important roles in cell differentiation, proliferation, and migration. Abnormal expression, mutation,…”
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Preserving single cells in space and time for analytical assays by Gallion, Luke A., Anttila, Matthew M., Abraham, David H., Proctor, Angela, Allbritton, Nancy L.

“…Analytical assays performed within clinical laboratories influence roughly 70% of all medical decisions by facilitating disease detection, diagnosis, and…”
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Co-fabrication of chitosan and epoxy photoresist to form microwell arrays with permeable hydrogel bottoms by Ornoff, Douglas M, Wang, Yuli, Proctor, Angela, Shah, Akash S, Allbritton, Nancy L

“…Abstract Microfabrication technology offers the potential to create biological platforms with customizable patterns and surface chemistries, allowing precise…”
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Metabolism of peptide reporters in cell lysates and single cells by Proctor, Angela, Wang, Qunzhao, Lawrence, David S, Allbritton, Nancy L

“…The stability of an Abl kinase substrate peptide in a cytosolic lysate and in single cells was characterized. In the cytosolic lysate, the starting peptide was…”
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Development of a Peptidase-Resistant Substrate for Single-Cell Measurement of Protein Kinase B Activation by Proctor, Angela, Wang, Qunzhao, Lawrence, David S, Allbritton, Nancy L

“…An iterative design strategy using three criteria was utilized to develop a peptidase-resistant substrate peptide for protein kinase B. Libraries of peptides…”
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Development of a protease-resistant reporter to quantify BCR-ABL activity in intact cells by Proctor, Angela, Zigoneanu, Imola G, Wang, Qunzhao, Sims, Christopher E, Lawrence, David S, Allbritton, Nancy L

“…A peptidase-resistant ABL kinase substrate was developed by identifying protease-susceptible bonds on an ABL substrate peptide and replacing flanking amino…”
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Using Follow-Up Coaching to Increase Self-Efficacy in Foster and Adoptive Parents Using Trauma-Informed Approaches with Their Children by Proctor, Angela M

“…Thousands of parents make the decision to foster and/or adopt children who have suffered various forms of abuse and neglect. There are hundreds of thousands of…”
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Abstract 3345: Measurement of Akt activity in single primary human pancreatic cancer cells using chemical cytometry by Proctor, Angela, Herrera-Loeza, S. Gabriela, Yeh, Jen Jen, Allbritton, Nancy L.

“…Genetic alterations in signaling pathways and processes, including mutations, deletions, and amplifications, have been found in many pancreatic cancers…”
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Construction of Peptidase-Resistant Substrates for Kinases by Proctor, Angela, Wang, Qunzhao, Lawrence, David S., Allbritton, Nancy L.

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Rational Design of a Dephosphorylation-Resistant Reporter Enables Single-Cell Measurement of Tyrosine Kinase Activity by Turner, Abigail H, Lebhar, Michael S, Proctor, Angela, Wang, Qunzhao, Lawrence, David S, Allbritton, Nancy L

“…Although peptide-based reporters of protein tyrosine kinase (PTK) activity have been used to study PTK enzymology in vitro, the application of these reporters…”
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β-Turn sequences promote stability of peptide substrates for kinases within the cytosolic environment by Yang, Shan, Proctor, Angela, Cline, Lauren L, Houston, Kaiulani M, Waters, Marcey L, Allbritton, Nancy L

“…A strategy was developed to extend the lifetime of an peptide-based substrate for Abl kinase in the cytosolic environment. Small β-turn structures were added…”
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Teachers' Ability to Detect Symptoms of Trauma in Children by Proctor, Angela M

“…This researcher explores trauma as a barrier to education and how teachers are able to detect it in students. The current society endures a high percentage of…”
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Development of Peptidase-Resistant Peptide Substrates for Measurement of Protein Kinase B and Bcr-Abl Kinase Activity by Proctor, Angela

“…Synthetic peptides are widely used by the biomedical research community as kinase substrates for purified kinases, in cell lysates, and sometimes in intact…”
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