Dengue Vaccines: Strongly Sought but Not a Reality Just Yet e1003551
The membrane protein (M) is synthesized as a precursor protein (prM) that is processed during export; it prevents E conformational changes from occurring during viral assembly. Besides these three structural proteins (C-prM-E), the viral genome codes for seven nonstructural proteins important for vi...
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| Published in: | PLoS pathogens Vol. 9; no. 10 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
San Francisco
Public Library of Science
01-10-2013
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | The membrane protein (M) is synthesized as a precursor protein (prM) that is processed during export; it prevents E conformational changes from occurring during viral assembly. Besides these three structural proteins (C-prM-E), the viral genome codes for seven nonstructural proteins important for viral replication, translation, and the proteolytic processing of the polyprotein. Vero cells are used to amplify the infectivity of the chimeric virus, which is formed by the respective DV serotype envelope proteins with the replication machinery (represented by gears) of an attenuated, related flavivirus. Since E is exposed on the virion surface, it contains the epitopes recognized by neutralizing antibodies and its gene is responsible for the genetic variation resulting in four DV serotypes [3]. Importantly, for both vaccines, a neutralization titer of at least 1:10 is protective. [...]the most useful and documented correlate of protection for flaviviruses is the presence of neutralizing antibodies assayed by the plaque reduction neutralization assay (PRNA). |
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| ISSN: | 1553-7366 1553-7374 |
| DOI: | 10.1371/journal.ppat.1003551 |