Form, synapses and orientation topography of a new cell type in layer 6 of the cat’s primary visual cortex

Here we report the morpho-functional features of a novel type of deep-layer neuron. The neuron was selected from a large pool of intracellularly labelled cells based on the large cell body, numerous spine-free dendrites with an overall interneuron morphology. However, the axon gave off long-range ax...

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Bibliographic Details
Published in:	Scientific reports Vol. 12; no. 1; p. 15428
Main Authors:	Srivastava, Mohit, Angel, Cintia, Kisvárday, Réka Eszter, Kocsis, Zsolt, Stelescu, András, Talapka, Petra, Kisvárday, Zoltán
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 14-09-2022 Nature Publishing Group Nature Portfolio
Subjects:	631/378/2613 631/378/2613/1875 631/378/3920 Cell body Cytology Dendrites Dendritic spines Electron microscopy Embryology Excavation Histology Human remains Humanities and Social Sciences Morphology multidisciplinary Neuroimaging Neurons Orientation behavior Presynapse Pyramidal cells Science Science (multidisciplinary) Software packages Spine Substantia alba Synaptogenesis Topography Visual cortex γ-Aminobutyric acid
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Summary:	Here we report the morpho-functional features of a novel type of deep-layer neuron. The neuron was selected from a large pool of intracellularly labelled cells based on the large cell body, numerous spine-free dendrites with an overall interneuron morphology. However, the axon gave off long-range axons up to 2.8 mm from the parent soma in layers 5/6 before entering the white matter. The boutons were uniformly distributed along the axon without forming distinct clusters. Dendritic length, surface area and volume values were at least 3 times larger than any known cortical neuron types with the exception of giant pyramidal cells of layer 5. Electron microscopy of the boutons revealed that they targeted dendritic spines (78%) and less frequently dendritic shafts (22%). Nearly half of the postsynaptic dendrites were immunopositive to GABA. Superimposing the axonal field on the orientation map obtained with optical imaging showed a preponderance of boutons to cross-orientations (38%) and an equal representation of iso- and oblique orientations (31%). The results suggest an integrating role for the layer 6 stellate neuron which projects to a functionally broad range of neurons in the deep cortical layers and to other cortical and/or subcortical regions.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-022-19746-9