Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs

Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs

Invasive is causally associated with granulomatous colitis (GC) of Boxer dogs and French Bulldogs. The virulence determinants of GC are unclear. isolated from 16 GC (36 strains) and 17 healthy control (HC: 33 strains) dogs were diverse in phylogeny, genotype, and serotype and lacked diarrheagenic ge...

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Bibliographic Details
Published in:Antibiotics (Basel) Vol. 9; no. 9; p. 540
Main Authors: Dogan, Belgin, Zhang, Shiying, Kalla, Sarah E, Dogan, Esra I, Guo, Cindy, Ang, Chelston R, Simpson, Kenneth W
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 25-08-2020
MDPI
Subjects:
adherent-invasive E. coli (AIEC)
Antigens
Autophagy
Boxer dogs
Causes of
Colitis
Cytotoxicity
Dog diseases
Dogs
E coli
Escherichia coli
fluoroquinolone
Genes
Genetic aspects
Genotype & phenotype
Genotypes
granulomatous colitis
Haplotypes
Identification and classification
Inflammation
Inflammatory bowel disease
Macrophages
monocyte derived macrophages
Monocytes
Motility
Mutation
Phylogeny
Physiological aspects
Pili
Strains (organisms)
Virulence
United States
granulomatous colitis
fluoroquinolone
CD48/SLAM
Escherichia coli
Boxer dogs
monocyte derived macrophages
adherent-invasive E. coli (AIEC)
very early onset inflammatory bowel disease (IBD)
Crohn’s disease
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Summary:Invasive is causally associated with granulomatous colitis (GC) of Boxer dogs and French Bulldogs. The virulence determinants of GC are unclear. isolated from 16 GC (36 strains) and 17 healthy control (HC: 33 strains) dogs were diverse in phylogeny, genotype, and serotype and lacked diarrheagenic genes. Genes encoding type II ( ), IV ( ), and VI ( ) secretion systems, long polar fimbriae ( 154/141), and iron acquisition ( , ) were frequent in GC and HC. from 14/15 GC and 10/11 HC invaded Caco-2 better than non-pathogenic strain DH5α, with invasion correlated with motility and presence of and . from all GC and 10/11 HC survived better than DH5α in J774 macrophages, with adherent-invasive (AIEC) in 60% GC and 73% HC. AIEC replicated in monocyte derived macrophages from a GC Boxer with CD48/SLAM risk haplotype but not the HC. Fluroquinolone resistant were less motile and invasive than fluoroquinolone sensitive ( < 0.05), and only 1/8 resistant strains met criteria for AIEC. In conclusion GC are diverse, resemble extraintestinal pathogenic (ExPEC), including AIEC, and can replicate in GC-susceptible macrophages. They are likely resident pathosymbionts that can opportunistically persist within macrophages of a GC-susceptible dog.
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ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics9090540