Identification and characterization of resistance and pathogenicity of Enterococcus spp . in samples of donor breast milk

Breast milk is the primary source of nutrition for newborns. Hospitalized babies frequently need nutritional support from Human Milk Banks. As bacterial species of the genus are part of the microbiota of healthy donors, they may contaminate samples of pumped breast milk. To identify and characterize...

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Bibliographic Details
Published in:	World journal of clinical pediatrics Vol. 9; no. 3; pp. 53 - 62
Main Authors:	Santana, Luana Andrade Mendes, Andrade, Nívea Nara Novais, da Silva, Lucas Santana Coelho, Oliveira, Caline Novais Teixeira, de Brito, Breno Bittencourt, de Melo, Fabrício Freire, Souza, Cláudio Lima, Marques, Lucas Miranda, Oliveira, Márcio Vasconcelos
Format:	Journal Article
Language:	English
Published:	United States Baishideng Publishing Group Inc 19-11-2020
Subjects:	Basic Study Human milk Antimicrobial resistance Virulence Enterococcus spp Pasteurization Breast milk
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Summary:	Breast milk is the primary source of nutrition for newborns. Hospitalized babies frequently need nutritional support from Human Milk Banks. As bacterial species of the genus are part of the microbiota of healthy donors, they may contaminate samples of pumped breast milk. To identify and characterize the bacterial virulence and resistance in samples isolated from the nipple-areolar region, hands, and breast milk aliquots from donors at the Human Milk Bank of Municipal Hospital Esaú Matos in the city of Vitória da Conquista, Bahia State, Brazil. The personal hygiene and sanitation of donors were analyzed with the aim of identifying possible reasons for contamination of pumped milk. Cutaneous samples as well as aliquots of unpasteurized and pasteurized milk from 30 participants were obtained. Each . isolate underwent a disk diffusion susceptibility test and molecular biology techniques to determine resistance and virulence genes. . were identified in 30% of donors ( = 9), and 11 specimens were isolated. Resistance to tetracycline was highly prevalent, being detectable in 63% of the isolates ( = 7) and followed by intermediate sensitivity to ciprofloxacin, observed in 27% of the specimens ( = 3). The A gene was found in 63% ( = 7) of the isolates, while the gene was detected in 27% ( = 3). This study illustrates the importance of microbiological monitoring by Human Milk Banks and the need for alternatives to prevent the presence of . in hospital settings.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Corresponding author: Fabrício Freire de Melo, MSc, PhD, Postdoc, Professor, Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Rua Hormindo Barros, 58, Quadra 17, Lote 58, Vitória da Conquista 45029-094, Bahia, Brazil. Author contributions: Santana LAM, Andrade NNN, da Silva LSC, Oliveira CNT, de Brito BB, de Melo FF, Souza CL, Marques LM, and Oliveira MV contributed to the conception and design of the study, literature review and analysis, drafting and critical revision and editing. All authors contributed equally to approval of the final version.
ISSN:	2219-2808 2219-2808
DOI:	10.5409/wjcp.v9.i3.53