Co-Functionalization of Gold Nanoparticles with C7H2 and HuAL1 Peptides: Enhanced Antimicrobial and Antitumoral Activities

Co-Functionalization of Gold Nanoparticles with C7H2 and HuAL1 Peptides: Enhanced Antimicrobial and Antitumoral Activities

The functionalization of nanoparticles with therapeutic peptides has been pointed out as a promising strategy to improve the applications of these molecules in the field of health sciences. Peptides are highly bioactive but face several limitations such as low bioavailability due to the difficulty o...

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Bibliographic Details
Published in:Pharmaceutics Vol. 14; no. 7; p. 1324
Main Authors: Formaggio, Daniela M. D., Magalhães, Jéssica A., Andrade, Vitor M., Conceição, Katia, Anastácio, Juliana M., Santiago, Gabrielli S., Arruda, Denise C., Tada, Dayane B.
Format: Journal Article
Language:English
Published: Basel MDPI AG 23-06-2022
MDPI
Subjects:
Antimicrobial agents
Aqueous solutions
C7H2
Cancer therapies
CDR peptides
Drug delivery systems
gold nanoparticles
HuaL1
Medical research
Melanoma
Metastasis
Nanoparticles
Nanotechnology
Peptides
Spectrum analysis
therapeutic peptides
tumor targeting
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Summary:The functionalization of nanoparticles with therapeutic peptides has been pointed out as a promising strategy to improve the applications of these molecules in the field of health sciences. Peptides are highly bioactive but face several limitations such as low bioavailability due to the difficulty of overcoming the physiological barriers in the body and their degradation by enzymes. In this work, gold nanoparticles (AuNPs) were co-functionalized with two therapeutic peptides simultaneously. The peptides from the complementary determining region of monoclonal antibodies, composed of the amino acid sequences YISCYNGATSYNQKFK (C7H2) and RASQSVSSYLA (HuAL1) were chosen for having exhibited antitumor and antimicrobial activity before. The peptides-conjugated AuNPs were characterized regarding size, morphology, and metal concentration by using TEM, dynamic light scattering, and ICP-OES techniques. Then, peptides-conjugated AuNPs were evaluated regarding the antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. The antitumoral activity was evaluated in vitro by cell viability assays with metastatic melanoma cell line (B16F10-Nex2) and the cytotoxicity was evaluated against human foreskin fibroblast (Hs68) cell line. Finally, in vivo assays were performed by using a syngeneic animal model of metastatic melanoma. Our findings have highlighted the potential application of the dual-peptide AuNPs in order to enhance the antitumor and antimicrobial activity of peptides.
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ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics14071324