Comparative conventional and phenomics approaches to assess symbiotic effectiveness of Bradyrhizobia strains in soybean (Glycine max L. Merrill) to drought

Comparative conventional and phenomics approaches to assess symbiotic effectiveness of Bradyrhizobia strains in soybean (Glycine max L. Merrill) to drought

Symbiotic effectiveness of rhizobitoxine (Rtx)-producing strains of Bradyrhizobium spp. in soybean (cultivar NRC-37/Ahilya-4) under limited soil moisture conditions was evaluated using phenomics tools such as infrared(IR) thermal and visible imaging. Red, green and blue (RGB) colour pixels were stan...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports Vol. 7; no. 1; pp. 6958 - 14
Main Authors: Govindasamy, Venkadasamy, George, Priya, Aher, Lalitkumar, Ramesh, Shunmugiah V., Thangasamy, Arunachalam, Anandan, Sivalingam, Raina, Susheel Kumar, Kumar, Mahesh, Rane, Jagadish, Annapurna, Kannepalli, Minhas, Paramjit Singh
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 31-07-2017
Nature Publishing Group
Nature Portfolio
Subjects:
38
38/22
38/23
38/39
631/326/2522
631/449/2661/2665
9/10
Bradyrhizobium
Bradyrhizobium - metabolism
Bradyrhizobium - physiology
Canopies
Correlation analysis
Cultivars
Drought resistance
Genotypes
Glycine max
Glycine max - growth & development
Glycine max - microbiology
Humanities and Social Sciences
Image processing
Infrared Rays
Inoculation
Light
multidisciplinary
Nodulation
Phenotype
Phenotyping
Photosynthesis
Plant Root Nodulation
Principal Component Analysis
Principal components analysis
Propanolamines - metabolism
Science
Science (multidisciplinary)
Soil moisture
Soybeans
Strains (organisms)
Symbiosis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Symbiotic effectiveness of rhizobitoxine (Rtx)-producing strains of Bradyrhizobium spp. in soybean (cultivar NRC-37/Ahilya-4) under limited soil moisture conditions was evaluated using phenomics tools such as infrared(IR) thermal and visible imaging. Red, green and blue (RGB) colour pixels were standardized to analyse a total of 1017 IR thermal and 692 visible images. Plants inoculated with the Rtx-producing strains B. elkanii USDA-61 and USDA-94 and successive inoculation by B. diazoefficiens USDA-110 resulted in cooler canopy temperatures and increased canopy greenness. The results of the image analysis of plants inoculated with Rtx-producing strains were correlated with effective nodulation, improved photosynthesis, plant nitrogen status and yield parameters. Principal component analysis (PCA) revealed the reliability of the phenomics approach over conventional destructive approaches in assessing the symbiotic effectiveness of Bradyrhizobium strains in soybean plants under watered (87.41–89.96%) and water-stressed (90.54–94.21%) conditions. Multivariate cluster analysis (MCA) revealed two distinct clusters denoting effective (Rtx) and ineffective (non-Rtx) Bradyrhizobium inoculation treatments in soybean. Furthermore, correlation analysis showed that this phenotyping approach is a dependable alternative for screening drought tolerant genotypes or drought resilience symbiosis. This is the first report on the application of non-invasive phenomics techniques, particularly RGB-based image analysis, in assessing plant-microbe symbiotic interactions to impart abiotic stress tolerance.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-06441-3