Rapid detection of multidrug-resistant Mycobacterium tuberculosis using the mycobacteria growth indicator tube (MGIT) system

The emergence of multidrug-resistant strains of Mycobacterium tuberculosis has increased the need for rapid drug susceptibility tests, which are needed for adequate patient treatment. The objective of the present study was to evaluate the mycobacteria growth indicator tube (MGIT) system to detect mu...

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Bibliographic Details
Published in:	Brazilian journal of medical and biological research Vol. 35; no. 10; pp. 1127 - 1131
Main Authors:	Telles, M A S, Bori, A, Amorim, A B R, Cruz, A F, Pini, M I T, Sato, D N
Format:	Journal Article
Language:	English
Published:	Brazil Associação Brasileira de Divulgação Científica 01-10-2002
Subjects:	Antitubercular Agents - pharmacology Bacteriological Techniques - methods BIOLOGY Culture Media Humans Isoniazid - pharmacology MEDICINE, RESEARCH & EXPERIMENTAL Microbial Sensitivity Tests - methods Multidrug resistance Multidrug-resistant Mycobacterium tuberculosis Mycobacteria growth indicator tube Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - growth & development Proportion method Resistance ratio method Rifampin - pharmacology Sensitivity and Specificity Susceptibility tests Time Factors Tuberculosis, Multidrug-Resistant - diagnosis Susceptibility tests Mycobacteria growth indicator tube Multidrug-resistant Mycobacterium tuberculosis Proportion method Multidrug resistance Resistance ratio method
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Summary:	The emergence of multidrug-resistant strains of Mycobacterium tuberculosis has increased the need for rapid drug susceptibility tests, which are needed for adequate patient treatment. The objective of the present study was to evaluate the mycobacteria growth indicator tube (MGIT) system to detect multidrug-resistant M. tuberculosis strains. The MGIT system was compared with two standard methods (proportion and resistance ratio methods). One hundred clinical M. tuberculosis isolates [25 susceptible to isoniazid (INH) and rifampicin (RIF), 20 resistant to INH, 30 resistant to INH-RIF, and 25 resistant to INH-RIF and other drugs] obtained in the State of S o Paulo were tested for INH and RIF susceptibility. Full agreement among the tests was found for all sensitive and all INH-resistant strains. For RIF-resistant strains results among the tests agreed for 53 (96.4%) of 55 isolates. Results were obtained within 6 days (range, 5 to 8 days), 28 days and 12 days when using MGIT, the proportion method and the resistance ratio methods, respectively. The MGIT system presented an overall agreement of 96% when compared with two standard methods. These data show that the MGIT system is rapid, sensitive and efficient for the early detection of multidrug-resistant M. tuberculosis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0100-879X 1414-431X 0100-879X 1414-431X
DOI:	10.1590/S0100-879X2002001000003