Expression of Fcγ and complement receptors in monocytes of X-linked agammaglobulinaemia and common variable immunodeficiency patients

Expression of Fcγ and complement receptors in monocytes of X-linked agammaglobulinaemia and common variable immunodeficiency patients

Recently we reported that monocyte phagocytosis and chemotaxis are impaired in X-linked agammaglobulinaemia (XLA) and common variable immunodeficiency (CVI) patients. Few data exist on the in vivo expression of receptors for the constant region of immunoglobulin (IgG) (FcγR) and complement receptors...

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Bibliographic Details
Published in:Clinical and experimental immunology Vol. 150; no. 3; pp. 422 - 428
Main Authors: Amoras, A.L.B, da Silva, M.T.N, Zollner, R.L, Kanegane, H, Miyawaki, T, Vilela, M.M.S
Format: Journal Article
Language:English
Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-12-2007
Blackwell Publishing Ltd
Blackwell
Blackwell Science Inc
Subjects:
Adolescent
Adult
Agammaglobulinemia - genetics
Agammaglobulinemia - immunology
Analytical, structural and metabolic biochemistry
Antigens, CD - blood
Biological and medical sciences
CD18) receptors
Child
Child, Preschool
common variable immunodeficiency
Common Variable Immunodeficiency - immunology
CR1 (CD35)
CR3 (CD11b
Fcγ
Female
flow cytometry
Fundamental and applied biological sciences. Psychology
Genetic Diseases, X-Linked - immunology
GPI-Linked Proteins
Humans
Immunophenotyping
Male
Molecular and cellular biology
Molecular biophysics
monocytes
Monocytes - immunology
Receptors, Complement - blood
Receptors, IgG - blood
Translational Studies
X-linked agammaglobulinaemia
Human
Immunopathology
Flow cytometry
monocytes
Common variable immunodeficiency
Monocyte
Immunoglobulin receptor
Biochemistry
Complement
CR1 (CD35)
Biophysics
FcγR receptor
Infantile sex linked agammaglobulinemia
CR3 CD11b
Fcγ
Molecular biology
CD18 receptors
X-linked agammaglobulinaemia
Biological receptor
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Summary:Recently we reported that monocyte phagocytosis and chemotaxis are impaired in X-linked agammaglobulinaemia (XLA) and common variable immunodeficiency (CVI) patients. Few data exist on the in vivo expression of receptors for the constant region of immunoglobulin (IgG) (FcγR) and complement receptors (CR) in these patients. The objective of this study was to investigate the expression of FcγR and CR on monocytes from XLA and CVI patients and compare it to that of healthy controls. Whole blood samples were obtained from 10 patients with XLA, 12 with CVI and 18 healthy controls. Monocyte phenotype was determined by flow cytometry with gating on CD14⁺ cells. Surface expression of FcγRI (CD64), FcγRII (CD32) and FcγRIII (CD16), CR1 (CD35) and CR3 (CD11b and CD18) was measured by determination of the proportion of CD14⁺ cells positive for each receptor and by receptor density. Compared to controls, a significantly higher percentage of CD16 and CD35⁺ monocytes from XLA (P = 0·002 and P = 0·007, respectively) were observed. The relative fluorescence intensity (RFI) expression of FcyRII (CD32) and FcyRIII (CD16) were significantly lower on CVI monocytes compared to controls (P = 0·001 and P = 0·035, respectively). XLA patients, who have a reduction of Bruton's tyrosine kinase (Btk), showed normal or increased percentages of monocytes expressing Fcy and complement receptors. CVI patients, who have normal expression of Btk, showed reduced expression of CD16 and CD32 on monocytes. Inefficient chemotaxis and phagocytosis, reported previously in XLA patients, could be due to defects of cytoplasmatic transduction mechanisms.
Bibliography:http://dx.doi.org/10.1111/j.1365-2249.2007.03512.x
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2007.03512.x