Evaluation of genetic differentiation among healthy and infected Buxus hyrcana with boxwood blight using RAPD and ISSR markers

Evaluation of genetic differentiation among healthy and infected Buxus hyrcana with boxwood blight using RAPD and ISSR markers

Background Buxus hyrcana (boxwood) is an endangered species in the Hyrcanian forests in the north of Iran. This tree is threatened by habitat loss but faces additional threats from the introduced disease the boxwood blight (caused by the fungus Calonectria pseudonaviculata syn. Cylindrocladium pseud...

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Bibliographic Details
Published in:New Zealand journal of forestry science Vol. 48; no. 1; pp. 1 - 10
Main Authors: Salehi Shanjani, Parvin, Javadi, Hamideh, Rasoulzadeh, Leila, Amirkhani, Mahmoud
Format: Journal Article
Language:English
Published: Cham Springer International Publishing 01-12-2018
SCION
Scion
Subjects:
Biomedical and Life Sciences
Blight
Buxaceae
Buxus
Buxus hyrcana
Climate change
Climate effects
Differentiation
Endangered species
Forestry
Fungi
Gene polymorphism
Gene sequencing
Genetic diversity
Genetic variability
Genetic variation
Genomes
Habitat loss
ISSR
Life Sciences
Markers
Plant propagation
Polymorphism
Population density
Population genetics
Population number
Population studies
Populations
Propagation
Random amplified polymorphic DNA
RAPD
Research Article
Seeds
Trees
Wildlife conservation
ISSR
Genetic variation
RAPD
Blight
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Summary:Background Buxus hyrcana (boxwood) is an endangered species in the Hyrcanian forests in the north of Iran. This tree is threatened by habitat loss but faces additional threats from the introduced disease the boxwood blight (caused by the fungus Calonectria pseudonaviculata syn. Cylindrocladium pseudonaviculatum, Cy. buxicola ) and the potential effects of climate change. As wide range of genetic polymorphism is necessary to ensure successful adaptation to rapid climatic changes. Methods Genetic diversity and differentiation between 15 healthy and 15 infected trees of each of two populations were studied using RAPD and ISSR molecular markers. Results High-band polymorphism was found in pooled samples of B. hyrcana using both ISSR (58%) and RAPD (73%) markers. The ISSR data and the combined data set classified the trees into two groups. However, data from RAPD clustered the trees into three groups. These results indicate different degrees of genetic variation in the sequences of the tested B. hyrcana genomes targeted by the two marker types used. Genetic variation was found to be relatively high, with most of the diversity occurring within populations. Analyses of healthy versus infected pooled samples based on both marker types indicated that genetic diversity parameters were mostly higher in healthy trees. Conclusions Boxwood blight has had a major effect on B. hyrcana , killing individual stems quickly especially in dense populations and reducing population size (as observed in all populations). Considerable within-population diversity, and higher genetic variability in healthy trees than infected ones, suggested conservation efforts should focus on survivor trees in each population and consider the establishment of tree reservations. Propagation of plants from seeds is preferred, since it would include the widest range of genetic variation.
ISSN:1179-5395
0048-0134
1179-5395
DOI:10.1186/s40490-018-0120-z