Strontium-Doped Bioglass-Laden Gelatin Methacryloyl Hydrogels for Vital Pulp Therapy

Strontium-Doped Bioglass-Laden Gelatin Methacryloyl Hydrogels for Vital Pulp Therapy

This study aimed to develop gelatin methacryloyl (GelMA)-injectable hydrogels incorporated with 58S bioactive glass/BG-doped with strontium for vital pulp therapy applications. GelMA hydrogels containing 0% (control), 5%, 10%, and 20% BG ( / ) were prepared. Their morphological and chemical properti...

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Bibliographic Details
Published in:Journal of functional biomaterials Vol. 15; no. 4; p. 105
Main Authors: Aminmansour, Sepideh, Gomes de Carvalho, Ana Beatriz, Medeiros Cardoso, Lais, Anselmi, Caroline, Rahimnejad, Maedeh, Dal-Fabbro, Renan, Benavides, Erika, Campos, Tiago Moreira Bastos, Borges, Alexandre Luiz Souto, Bottino, Marco C
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 01-04-2024
Subjects:
Adhesion
Biocompatibility
biocompatible materials
Bioglass
Biological activity
Biological products
Biomaterials
Biomedical materials
Cell adhesion
Cell viability
Chemical elements
Chemical properties
Degradation
Dental pulp
dental pulp capping
Dentin
Endodontics
Fourier transforms
Gelatin
Health aspects
Hydrogels
Infrared spectroscopy
Invoices
Mechanical properties
Mineralization
Nodules
Pulp
Scanning electron microscopy
Scientific equipment and supplies industry
Silica
Spectrum analysis
Strontium
Swelling ratio
Tissue engineering
Variance analysis
United States
Massachusetts
United States > US
biocompatible materials
hydrogels
bioglass
gelatin
dental pulp capping
strontium
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Summary:This study aimed to develop gelatin methacryloyl (GelMA)-injectable hydrogels incorporated with 58S bioactive glass/BG-doped with strontium for vital pulp therapy applications. GelMA hydrogels containing 0% (control), 5%, 10%, and 20% BG ( / ) were prepared. Their morphological and chemical properties were evaluated by scanning electron microscopy/SEM, energy dispersive spectroscopy/EDS, and Fourier transform infrared spectroscopy/FTIR (n = 3). Their swelling capacity and degradation ratio were also measured (n = 4). Cell viability (n = 8), mineralized matrix formation, cell adhesion, and spreading (n = 6) on DPSCs were evaluated. Data were analyzed using ANOVA/post hoc tests (α = 5%). SEM and EDS characterization confirmed the incorporation of BG particles into the hydrogel matrix, showing GelMA's (C, O) and BG's (Si, Cl, Na, Sr) chemical elements. FTIR revealed the main chemical groups of GelMA and BG, as ~1000 cm corresponds to Si-O and ~1440 cm to C-H. All the formulations were degraded by day 12, with a lower degradation ratio observed for GelMA+BG20%. Increasing the concentration of BG resulted in a lower mass swelling ratio. Biologically, all the groups were compatible with cells ( > 0.6196), and cell adhesion increased over time, irrespective of BG concentration, indicating great biocompatibility. GelMA+BG5% demonstrated a higher deposition of mineral nodules over 21 days ( < 0.0001), evidencing the osteogenic potential of hydrogels. GelMA hydrogels incorporated with BG present great cytocompatibility, support cell adhesion, and have a clinically relevant degradation profile and suitable mineralization potential, supporting their therapeutic potential as promising biomaterials for pulp capping.
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ISSN:2079-4983
2079-4983
DOI:10.3390/jfb15040105