IS6110 restriction fragment length polymorphism typing of Mycobacterium tuberculosis isolates from East Azerbaijan Province of Iran

IS6110 restriction fragment length polymorphism typing of Mycobacterium tuberculosis isolates from East Azerbaijan Province of Iran

To investigate the genetic variation among Mycobacterium tuberculosis isolates in the East Azerbaijan Province of Iran and to evaluate the level of and risk factors for recent transmission of tuberculosis (TB), we performed IS6110-based restriction fragment length polymorphism analysis of strains, i...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz Vol. 101; no. 5; pp. 517 - 521
Main Authors: Asgharzadeh, Mohamad, Shahbabian, Karen, Majidi, Jafar, Aghazadeh, Ahmad Mirza, Amini, Cirus, Jahantabi, Ali Reza, Rafi, Abdolnaser
Format: Journal Article
Language:English
Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01-08-2006
Instituto Oswaldo Cruz, Ministério da Saúde
Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:
Adult
Bacterial Typing Techniques - methods
Cluster Analysis
Female
Genetic Variation
Humans
Iran
IS6110
Male
Middle Aged
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
PARASITOLOGY
Polymorphism, Restriction Fragment Length
restriction fragment length polymorphism
Risk Factors
TROPICAL MEDICINE
tuberculosis
Tuberculosis - microbiology
tuberculosis - restriction fragment length polymorphism - IS6110 - Iran
Tuberculosis - transmission
Iran
IS6110
restriction fragment length polymorphism
Iran
tuberculosis
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Summary:To investigate the genetic variation among Mycobacterium tuberculosis isolates in the East Azerbaijan Province of Iran and to evaluate the level of and risk factors for recent transmission of tuberculosis (TB), we performed IS6110-based restriction fragment length polymorphism analysis of strains, isolated from 105 patients during the period of September 2002 to March 2003 in TB centers and university hospitals of the province. Among 105 isolates, 81 different IS6110 patterns were found, of which 70 were observed only once and 11 were shared by two to eight isolates. Ninety-six isolates (91.4%) were found to have more than five copies of IS6110 and together with high patterns polymorphism, shows that IS6110-RFLP typing could be useful for studying the epidemiology of TB in Azerbaijan. The minimum estimated rate of recent transmission was 23%, suggesting that the degree of recent transmission in East Azerbaijan Province is relatively low. Clustering was not associated with age, sex or site of infection of TB but drug-resistant isolates were less likely to be clustered than sensitive isolates (p < 0.05).
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ISSN:1678-8060
0074-0276
0074-0276
1678-8060
DOI:10.1590/S0074-02762006000500006