Antibacterial activity of green synthesized copper oxide nanoparticles against multidrug-resistant bacteria

Using plant extracts in the green synthesis of nanoparticles has become an environmentally acceptable approach. In our study, copper oxide nanoparticles (CuO NPs) were synthesized using ethanolic extracts of Azadirachta indica and Simmondsia chinensis . CuO NP formation was confirmed by the change i...

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Bibliographic Details
Published in:	Scientific reports Vol. 14; no. 1; pp. 25020 - 21
Main Authors:	Khairy, Toka, Amin, Dina Hatem, Salama, Hanaa Mohamed, Elkholy, Iman Mohamed Amin, Elnakib, Mostafa, Gebreel, Hassan Mahmoud, Sayed, Hayam Abd Elnabi
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 23-10-2024 Nature Publishing Group Nature Portfolio
Subjects:	631/326 639/925 Acinetobacter Anti-Bacterial Agents - chemical synthesis Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibacterial activity Antibiotics Azadirachta - chemistry Azadirachta indica Biofilms - drug effects Clinical isolates Copper Copper - chemistry Copper - pharmacology Copper oxide nanoparticle Drug resistance Drug Resistance, Multiple, Bacterial - drug effects E coli Escherichia coli Escherichia coli - drug effects Green Chemistry Technology Healthcare-associated infection Humanities and Social Sciences Humans Klebsiella pneumoniae Klebsiella pneumoniae - drug effects Metal Nanoparticles - chemistry Methicillin Methicillin-Resistant Staphylococcus aureus - drug effects Microbial Sensitivity Tests Minimum inhibitory concentration Molecular Docking Simulation multidisciplinary Multidrug resistance Multidrug-resistant bacteria Nanoparticles Penicillin Penicillin-binding protein Plant extract Plant extracts Plant Extracts - chemistry Plant Extracts - pharmacology Pseudomonas aeruginosa Science Science (multidisciplinary) Simmondsia chinensis Spectroscopy Staphylococcus aureus Staphylococcus infections Stenotrophomonas maltophilia β Lactamase Plant extract Antibacterial activity Healthcare-associated infection Copper oxide nanoparticle Multidrug-resistant bacteria
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Summary:	Using plant extracts in the green synthesis of nanoparticles has become an environmentally acceptable approach. In our study, copper oxide nanoparticles (CuO NPs) were synthesized using ethanolic extracts of Azadirachta indica and Simmondsia chinensis . CuO NP formation was confirmed by the change in color and by UV‒visible spectroscopy (CuO NPs peaked at a wavelength of 344 nm). TEM images confirmed the semispherical shape of the CuO NPs, with particle sizes ranging from 30.9 to 10.7 nm. The antibacterial activity of these NPs was evaluated by using the agar diffusion method against clinical isolates, including methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli , Pseudomonas aeruginosa , Acinetobacter spp., Klebsiella pneumoniae , and Stenotrophomonas maltophilia . The minimum inhibitory concentration (MIC) of CuO NPs ranged from 62.5 to 125 µg/ml. In contrast, the antioxidant activity and antibiofilm activity of CuO NPs ranged from 31.1 to 92.2% at 125–500 µg/ml and 62.2–95%, respectively, at 125 –62.5 µg/ml. Our results confirmed that CuO NPs had IC50s of 383.41 ± 3.4 and 402.73 ± 1.86 at 250 µg/mL against the HBF4 cell line. Molecular docking studies with CuO NPs suggested that penicillin-binding protein 4 (PBP4) and beta-lactamase proteins (OXA-48) strongly bind to S. aureus and K. pneumoniae , respectively, with CuO NPs. Our study confirms the promising use of CuO NPs in treating pathogenic bacteria and that CuO NPs could be possible alternative antibiotics. This study supports the pharmaceutical and healthcare sectors in Egypt and worldwide.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-024-75147-0